Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences, Shijiazhuang, China.
School of Resources and Environmental Engineering, Hefei University of Technology, Hefei, China.
Water Environ Res. 2024 Aug;96(8):e11078. doi: 10.1002/wer.11078.
Petroleum hydrocarbons (PHCs) can be biodegraded into CO, and PHC-contaminated aquifers are always deemed as carbon sources. Fortunately, some carbon fixation microorganisms have been found in PHC-contaminated sites. However, most of the studies are related to volatile short-chain PHC, and few studies focus on long-chain PHC-contaminated sites. To reveal the carbon fixation microorganisms in these sites, in the study, a long-chain PHC polluted site in North China was selected. Through hydrochemical and metagenomics analysis, the structure and capacity of carbon fixing microorganisms in the site were revealed. Results showed that there were many kinds of carbon fixed microorganisms that were identified such as Flavobacterium, Pseudomonas. HP/4HB, rTCA, and DC/4HB cycles were dominated carbon fixation pathways. The long-chain PHC were weakly correlated with carbon fixation microorganisms, but it may stimulate the growth of some carbon fixation microorganisms, such as microorganisms involved in rTCA cycle. PRACTITIONER POINTS: The microorganisms with carbon fixation gene exist in the aquifer contaminated by long-chain petroleum hydrocarbon. Microorganisms that have the ability to degrade petroleum also have the ability to carbon fixation. Long-chain petroleum hydrocarbon may promote the growth of carbon fixation microorganisms.
石油烃 (PHC) 可生物降解为 CO，受 PHC 污染的含水层通常被视为碳源。幸运的是，在受 PHC 污染的地点已经发现了一些碳固定微生物。然而，大多数研究都与挥发性短链 PHC 有关，很少有研究关注长链 PHC 污染的地点。为了揭示这些地点的碳固定微生物，在本研究中，选择了中国北方的一个长链 PHC 污染地点。通过水化学和宏基因组学分析，揭示了该地点碳固定微生物的结构和能力。结果表明，存在许多种已鉴定的碳固定微生物，如黄杆菌、假单胞菌。HP/4HB、rTCA 和 DC/4HB 循环是主要的碳固定途径。长链 PHC 与碳固定微生物的相关性较弱，但它可能会刺激某些碳固定微生物的生长，例如参与 rTCA 循环的微生物。
带有碳固定基因的微生物存在于受长链石油烃污染的含水层中。具有石油降解能力的微生物也具有碳固定能力。长链石油烃可能会促进碳固定微生物的生长。
