Ma Guangwei, Zuo Ziye, Li Handong, Zhai Xiaofei, Wang Tongliang, Wang Jichao
Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, China.
PLoS One. 2024 Aug 1;19(8):e0305858. doi: 10.1371/journal.pone.0305858. eCollection 2024.
Exposure to noise can cause non-auditory health problems and has been widely studied in mammals such as rats and rabbits. However, the non-auditory effects of noise exposure on reptiles (such as red-eared sliders) remain unclear. In this study, we determined the noise exposure-induced transcriptomic changes in the liver of red-eared slider (Trachemys scripta elegans) using Illumina Novaseq6000 sequencing technology. The transcriptome analysis identified 176 differentially expressed genes (DEGs), which were mainly enriched in lipid metabolism. KEGG analysis showed that by affecting the peroxisome proliferator activated receptor (PPAR) signaling pathway these DEGs increased lipid synthesis and decreased lipid oxidation. The Oil Red O staining results validated our data that noise exposure increased hepatic lipid deposition. Thus, noise exposure may lead to lipid accumulation and toxicity, mitochondrial damage, and accelerated oxidative stress. Our findings provide insights into the molecular process underlying non-auditory damage caused by noise exposure in T. scripta elegans.
接触噪音会导致非听觉性健康问题，并且在大鼠和兔子等哺乳动物中已得到广泛研究。然而，噪音暴露对爬行动物（如红耳龟）的非听觉影响仍不清楚。在本研究中，我们使用Illumina Novaseq6000测序技术确定了噪音暴露引起的红耳龟（滑龟指名亚种）肝脏转录组变化。转录组分析鉴定出176个差异表达基因（DEGs），这些基因主要富集于脂质代谢。KEGG分析表明，这些DEGs通过影响过氧化物酶体增殖物激活受体（PPAR）信号通路增加了脂质合成并减少了脂质氧化。油红O染色结果验证了我们的数据，即噪音暴露增加了肝脏脂质沉积。因此，噪音暴露可能导致脂质积累和毒性、线粒体损伤以及加速氧化应激。我们的研究结果为滑龟指名亚种中噪音暴露引起的非听觉损伤的分子过程提供了见解。
