Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China.
Joint Laboratory for Monitoring and Conservation of Aquatic Living Resources in the Yangtze Estuary, Shanghai 200092, China; Shanghai Aquatic Wildlife Conservation and Research Center, Shanghai 200003, China.
Sci Total Environ. 2022 Dec 10;851(Pt 2):158348. doi: 10.1016/j.scitotenv.2022.158348. Epub 2022 Aug 30.
Underwater noise from ship engines can affect the metabolism and immune system of various fish species. Meanwhile, changes in the metabolic pathways in liver are important for fish to adapt to adverse environments. We used a combined multi-omics analysis to investigate the response mechanism of hybrid sturgeon to continuously played ship noise. A control group and a noise group (simulated ship noise: 12 h) were set up, and liver tissues were extracted for high-throughput transcriptome and metabolome sequencing. The results show that a total of 588 differentially expressed genes (DEGs) and 58 DEGs metabolites were detected. The joint analysis of transcriptome and metabolome showed that under noise stress, apoptosis and cell motility were intensified, DNA replication, RNA transcription and translation, and protein synthesis were inhibited, and lipid metabolism, nucleotide metabolism, and vitamin D3 metabolic pathways were also inhibited. Interestingly, the initiation of a partial immune responses ensured their normal immunity abilities. Moreover, material and energy requirements of the organism under noise stress were guaranteed by upregulation of carbohydrate and amino acid metabolic pathways.
船舶引擎产生的水下噪音会影响各种鱼类的新陈代谢和免疫系统。同时，肝脏代谢途径的变化对于鱼类适应不利环境非常重要。我们使用联合多组学分析方法来研究杂交鲟对持续播放的船舶噪音的反应机制。设置了对照组和噪音组（模拟船舶噪音：12 小时），并提取肝脏组织进行高通量转录组和代谢组测序。结果表明，共检测到 588 个差异表达基因（DEGs）和 58 个 DEGs 代谢物。转录组和代谢组的联合分析表明，在噪声应激下，细胞凋亡和运动性增强，DNA 复制、RNA 转录和翻译以及蛋白质合成受到抑制，脂质代谢、核苷酸代谢和维生素 D3 代谢途径也受到抑制。有趣的是，部分免疫反应的启动确保了它们正常的免疫能力。此外，通过上调碳水化合物和氨基酸代谢途径，保证了机体在噪声应激下的物质和能量需求。
