Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, 88 Jiaotong Road, Fuzhou, Fujian, 350004, China.
Institute of Stomatology & Research Center of Dental Esthetics and Biomechanics, School and Hospital of Stomatology, Fujian Medical University, 246 Yangqiao Zhong Road, Fuzhou, Fujian, 350002, China.
J Mech Behav Biomed Mater. 2024 Oct;158:106674. doi: 10.1016/j.jmbbm.2024.106674. Epub 2024 Jul 24.
Alveolar bone defects caused by tumor, trauma and inflammation can lead to the loss of oral function and complicate denture restoration. Currently, guided bone regeneration (GBR) barrier membranes for repairing bone defect cannot effectively promote bone regeneration due to their unstable degradation rate and poor antibacterial properties. Furthermore, they require additional tailoring before implantation. Therefore, this study developed a visible light-curing hydrogel membrane (CF-Cu) comprising methacrylated carboxymethyl chitosan (CMCS-MA), silk fibroin (SF), and copper nanoparticles (Cu NPs) to address these shortcomings of commercial membranes. The CF-Cu hydrogel, characterized by scanning electron microscopy (SEM), a universal testing machine, and swelling and degradation tests, demonstrated a smooth porous network structure, suitable swelling ratio, biodegradability, and enhanced mechanical strength. Cytotoxicity and hemolysis tests in vitro demonstrated excellent cyto- and hemo-compatibility of the CF-Cu hydrogel extracts. Additionally, evaluation of antibacterial properties in vitro, including colony forming unit (CFU) counts, MTT assays, and live/dead fluorescence staining, showed that the CF-Cu hydrogel exhibited excellent antibacterial properties, inhibiting over 80% of S. aureus, S. mutans, and P. gingivalis with CF-1Cu hydrogel compared to the control group. Moreover, evaluation of osteogenic differentiation of rBMSCs in vitro suggested that the CF-1Cu hydrogel significantly improved alkaline phosphatase (ALP) activity and the mineralization of extracellular matrix, up-regulating the expressions of osteogenesis-related genes (Runx2, ALP, Col-1, OPN and BSP). In summary, these results indicated that CF-1Cu hydrogel exhibited excellent cytocompatibility, antibacterial and osteogenic properties in vitro. Therefore, the CF-1Cu hydrogel holds potential as a viable material for application in GBR procedures aimed at addressing bone defects.
肿瘤、外伤和炎症引起的牙槽骨缺损可导致口腔功能丧失，并使义齿修复复杂化。目前，用于修复骨缺损的引导骨再生（GBR）屏障膜由于其降解率不稳定和抗菌性能差，不能有效地促进骨再生。此外，在植入前还需要进行额外的裁剪。因此，本研究开发了一种可见光固化水凝胶膜（CF-Cu），该水凝胶膜由甲基丙烯酰化羧甲基壳聚糖（CMCS-MA）、丝素蛋白（SF）和铜纳米粒子（Cu NPs）组成，以解决商业膜的这些缺点。CF-Cu 水凝胶通过扫描电子显微镜（SEM）、万能试验机、溶胀和降解试验进行了表征，具有光滑的多孔网络结构、合适的溶胀比、生物降解性和增强的机械强度。体外细胞毒性和溶血试验表明 CF-Cu 水凝胶提取物具有优异的细胞和血液相容性。此外，体外抗菌性能评价，包括菌落形成单位（CFU）计数、MTT 测定和活/死荧光染色，表明 CF-Cu 水凝胶具有优异的抗菌性能，与对照组相比，CF-1Cu 水凝胶对金黄色葡萄球菌、变形链球菌和牙龈卟啉单胞菌的抑制率超过 80%。此外，体外 rBMSCs 成骨分化评价表明，CF-1Cu 水凝胶显著提高碱性磷酸酶（ALP）活性和细胞外基质矿化，上调成骨相关基因（Runx2、ALP、Col-1、OPN 和 BSP）的表达。综上所述，这些结果表明 CF-1Cu 水凝胶在体外具有优异的细胞相容性、抗菌和成骨性能。因此，CF-1Cu 水凝胶作为一种可行的 GBR 程序应用材料具有潜力，可用于治疗骨缺损。
