Fauzi Muhammad Syazwan Ahmad, Sabri Muhammad Syafiq Ahmad, Halim Adyani Azizah Abd, Abidin Siti Amalina Inche Zainal
Faculty of Dentistry, Universiti Malaya, Wilayah Persekutuan Kuala Lumpur, Malaysia.
Department of Oral and Craniofacial Sciences, Faculty of Dentistry, Universiti Malaya, Wilayah Persekutuan Kuala Lumpur, Malaysia.
J Stomatol Oral Maxillofac Surg. 2025 Feb;126(1):101999. doi: 10.1016/j.jormas.2024.101999. Epub 2024 Jul 30.
Medication-related osteonecrosis of the jaw (MRONJ) is a severe complication associated with prolonged bisphosphonate therapy. Increasing evidence shows that mucosal damage plays an important role in the pathogenesis of MRONJ. This study investigates the combinatorial effects of hydroxyapatite with Tualang honey on cell viability and wound healing in MRONJ.
The incorporation of Tualang honey into hydroxyapatite was assessed using Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and field emission scanning electron-energy dispersive X-ray analysis microscopy (FESEM-EDX). The effect of hydroxyapatite combined with Tualang honey on cell viability was determined by WST-1 assay and wound healing was assessed by scratch assay.
The incorporation of Tualang honey into hydroxyapatite altered the functional groups, structure, size, morphology, and components of the crystal as evidenced by FTIR, XRD and FESEM-EDX analysis. High concentrations of pamidronic acid inhibit oral fibroblast viability and wound healing. Low and high concentrations of hydroxyapatite demonstrate non-toxicity towards fibroblast cells. Furthermore, hydroxyapatite reversed the action of pamidronic acid on the cells; it increased fibroblast viability but did not close the wound. Tualang honey promotes fibroblast viability and wound closure. However, the addition of Tualang honey is unable to overcome the inhibitory effects of pamidronic acid on fibroblasts. The addition of Tualang honey and hydroxyapatite improved the cell viability and accelerated wound closure of fibroblast exposed to pamidronic acid.
These findings demonstrated that the combination treatment protects oral fibroblasts by preventing bisphosphonate toxicity.
药物相关性颌骨坏死（MRONJ）是一种与长期双膦酸盐治疗相关的严重并发症。越来越多的证据表明，黏膜损伤在MRONJ的发病机制中起重要作用。本研究调查了羟基磷灰石与杜兰蜂毒蜜对MRONJ中细胞活力和伤口愈合的联合作用。
使用傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）和场发射扫描电子能谱X射线分析显微镜（FESEM-EDX）评估杜兰蜂毒蜜在羟基磷灰石中的掺入情况。通过WST-1法测定羟基磷灰石与杜兰蜂毒蜜联合对细胞活力的影响，并通过划痕试验评估伤口愈合情况。
FTIR、XRD和FESEM-EDX分析表明，杜兰蜂毒蜜掺入羟基磷灰石改变了晶体的官能团、结构、大小、形态和成分。高浓度的帕米膦酸抑制口腔成纤维细胞活力和伤口愈合。低浓度和高浓度的羟基磷灰石对成纤维细胞均无毒性。此外，羟基磷灰石逆转了帕米膦酸对细胞的作用；它增加了成纤维细胞活力，但未闭合伤口。杜兰蜂毒蜜促进成纤维细胞活力和伤口闭合。然而，添加杜兰蜂毒蜜无法克服帕米膦酸对成纤维细胞的抑制作用。添加杜兰蜂毒蜜和羟基磷灰石可提高暴露于帕米膦酸的成纤维细胞的活力并加速伤口闭合。
这些发现表明联合治疗可通过预防双膦酸盐毒性来保护口腔成纤维细胞。
