Kawakami F, Okamura H, Fukui K, Yanaihara C, Yanaihara N, Nakajima T, Ibata Y
Neurosci Lett. 1985 Nov 11;61(3):273-7. doi: 10.1016/0304-3940(85)90476-8.
The influence of serotonin (5-hydroxytryptamine; 5-HT) innervation on peptide-containing neurons in the rat suprachiasmatic nucleus (SCN) was investigated by peroxidase-anti-peroxidase (PAP) immunocytochemistry. The 5-HT neuronal system was chemically severed by 5,6-dihydroxytryptamine (5,6-DHT) injection into the medial forebrain bundle bilaterally. After this treatment, a marked decrease of vasoactive intestinal peptide (VIP)-like immunoreactivity in neuronal perikarya occurred in the SCN corresponding to a decrease in number of 5-HT immunoreactive fibers and terminals. However, no alteration of arginine-vasopressin-like immunoreactivity was detected between 5,6-DHT-treated animals and the controls. It is speculated that VIP-like immunoreactive neurons play an important role in the SCN under the influence of strong 5-HT innervation.
通过过氧化物酶抗过氧化物酶（PAP）免疫细胞化学方法，研究了5-羟色胺（血清素；5-HT）神经支配对大鼠视交叉上核（SCN）中含肽神经元的影响。通过双侧向内侧前脑束注射5,6-二羟基色胺（5,6-DHT），化学切断5-HT神经元系统。经过这种处理后，SCN中神经元胞体的血管活性肠肽（VIP）样免疫反应性显著降低，这与5-HT免疫反应性纤维和终末数量的减少相对应。然而，在5,6-DHT处理的动物和对照组之间，未检测到精氨酸加压素样免疫反应性的改变。推测VIP样免疫反应性神经元在强大的5-HT神经支配影响下，在SCN中发挥重要作用。
