Kiss J, Léránth C, Halász B
Neurosci Lett. 1984 Feb 10;44(2):119-24. doi: 10.1016/0304-3940(84)90068-5.
A combination of immunocytochemistry with transmitter specific autoradiography at the electron microscopic level was used to identify vasoactive intestinal polypeptide (VIP)- or ACTH-containing neurons and serotoninergic nerve terminals in the suprachiasmatic (SCN) and arcuate nuclei (AN). We observed nerve terminals showing selective uptake of [3H]serotonin forming synaptic contact with perikarya or dendrites where the postsynaptic structures exhibited VIP-like immunoreactivity in the SCN and ACTH-like immunoreactivity in the AN. The findings provide ultrastructural evidence that serotoninergic nerve fibers terminate on VIP-neurons in the SCN and on ACTH-neurons in the AN.
在电子显微镜水平上，将免疫细胞化学与递质特异性放射自显影相结合，用于鉴定视交叉上核（SCN）和弓状核（AN）中含血管活性肠肽（VIP）或促肾上腺皮质激素（ACTH）的神经元以及5-羟色胺能神经末梢。我们观察到，显示对[3H]5-羟色胺有选择性摄取的神经末梢与核周体或树突形成突触联系，其中突触后结构在SCN中表现出VIP样免疫反应性，在AN中表现出ACTH样免疫反应性。这些发现提供了超微结构证据，表明5-羟色胺能神经纤维终止于SCN中的VIP神经元和AN中的ACTH神经元。
