Scarbrough Daniel, Bartels Randy, Squier Jeff
Opt Lett. 2024 Aug 1;49(15):4373-4376. doi: 10.1364/OL.531471.
Spatial frequency modulation imaging (SPIFI) has previously been demonstrated in multiphoton modalities with resolution enhancement. When signal light levels are low enough, signals consist of discrete pulses: this is a photon-counting regime. By binning photon counts into discrete time bins, SPIFI signals can be built up by incrementing the photon counts over many modulation periods. The result is an increase in the signal-to-noise ratio (SNR) over analog SPIFI acquisition, enabling higher fidelity in the greater orders of resolution enhancement that SPIFI can produce.
空间频率调制成像（SPIFI）此前已在具有分辨率增强功能的多光子模态中得到验证。当信号光水平足够低时，信号由离散脉冲组成：这是一个光子计数模式。通过将光子计数合并到离散的时间区间内，SPIFI信号可以通过在多个调制周期内增加光子计数来构建。结果是与模拟SPIFI采集相比，信噪比（SNR）有所提高，从而在SPIFI能够实现的更高分辨率增强阶数上实现更高的保真度。
