Kharamani Afshin, Mashayekhi Farhad, Salehi Zivar
Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
J Hum Reprod Sci. 2024 Apr-Jun;17(2):121-127. doi: 10.4103/jhrs.jhrs_68_24. Epub 2024 Jun 28.
Fibroblast growth factors (FGFs) play a key role in embryo implantation and support endometrial trophoblastic interaction.
The aim of the study was to evaluate the association between FGF-1 (rs34011) gene variety and its serum concentration with repeated implantation failure (RIF).
The design of the study was a cross-sectional study.
Four hundred infertile women with a history of RIF and 400 healthy women undergoing the first fertilisation-embryo transfer attempt with successful delivery (controls) were enrolled in the study. Genomic DNA was extracted from peripheral blood leucocytes and genotyped by Tetra-Primer Amplification Refractory Mutation System-Polymerase Chain Reaction. Serum FGF-1 concentration was evaluated with enzyme-linked immunosorbent assay.
The ANOVA test was used to analyse the difference between the means of the groups.
In RIF group, the genotype frequencies of the GG, GA and AA were 59%, 33.5% and 7.5%, respectively, whereas in controls were 72.5%, 24% and 3.5%, respectively. The G and A allele frequencies in the RIF group were 75.75% and 24.25%, while in controls were 84.5% and 15.5%, respectively ( < 0.0001). We have also shown that serum FGF-1 concentration in RIF and control groups was 17 ± 3.55 and 23.62 ± 4.91 pg/mL, respectively ( = 0.008). We have also shown that AA genotype is significantly associated with decreased serum FGF-1 concentration in RIF (AA, GA and GG serum levels were 9.55 ± 2.65, 14 ± 3.35 and 22.55 ± 7.26 pg/mL, and in controls were 12.22 ± 2.27, 18.44 ± 5.98 and 26.66 ± 8.29 pg/mL, respectively).
The current study suggests that a significant association between FGF-1 (rs34011) promoter polymorphism and its serum concentration with RIF. The study also suggests that AA genotype is linked to lower FGF-1 serum levels and may play a risk factor for RIF.
成纤维细胞生长因子（FGFs）在胚胎着床及支持子宫内膜与滋养层细胞相互作用中起关键作用。
本研究旨在评估FGF-1（rs34011）基因变异及其血清浓度与反复种植失败（RIF）之间的关联。
本研究设计为横断面研究。
400例有反复种植失败病史的不孕女性和400例首次接受受精-胚胎移植并成功分娩的健康女性（对照组）纳入本研究。从外周血白细胞中提取基因组DNA，并采用四引物扩增阻滞突变系统-聚合酶链反应进行基因分型。采用酶联免疫吸附测定法评估血清FGF-1浓度。
采用方差分析检验分析各组均值之间的差异。
在反复种植失败组中，GG、GA和AA基因型频率分别为59%、33.5%和7.5%，而在对照组中分别为72.5%、24%和3.5%。反复种植失败组中G和A等位基因频率分别为75.75%和24.25%，而在对照组中分别为84.5%和15.5%（P<0.0001）。我们还发现，反复种植失败组和对照组的血清FGF-1浓度分别为17±3.55和23.62±4.91 pg/mL（P=0.008）。我们还发现，AA基因型与反复种植失败组血清FGF-1浓度降低显著相关（AA、GA和GG血清水平分别为9.55±2.65、14±3.35和22.55±7.26 pg/mL，而在对照组中分别为12.22±2.27、18.44±5.98和26.66±8.29 pg/mL）。
本研究表明，FGF-1（rs34011）启动子多态性及其血清浓度与反复种植失败之间存在显著关联。该研究还表明，AA基因型与较低的FGF-1血清水平相关，可能是反复种植失败的一个危险因素。
