Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, China.
Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry, Guangzhou, Guangdong, China.
Front Immunol. 2024 Jul 18;15:1452609. doi: 10.3389/fimmu.2024.1452609. eCollection 2024.
Galectins (Gals) are a type of S-type lectin that are widespread and evolutionarily conserved among metazoans, and can act as pattern recognition receptors (PRRs) to recognize pathogen-associated molecular patterns (PAMPs). In this study, () were identified in the Golden pompano (), and their conserved domains, motifs, and collinearity relationships were analyzed. The expression of was regulated following infection to and , indicating that participate in immune responses against microbial pathogens. Further analysis was conducted on one important member, Galectin-3, subcellular localization showing that ToGal-3like protein is expressed both in the nucleus and cytoplasm. Recombinant protein obtained through prokaryotic expression showed that rToGal-3like can agglutinate red blood cells of rabbit, carp and golden pompano and also agglutinate and kill , and . This study lays the foundation for further research on the immune roles of in teleosts.
半乳糖凝集素（Galectins，Gals）是 S 型凝集素的一种，广泛存在于后生动物中，在进化上具有保守性，可作为模式识别受体（Pattern recognition receptors，PRRs）识别病原体相关分子模式（Pathogen-associated molecular patterns，PAMPs）。本研究在金鲳（Trachinotus ovatus）中鉴定了（半乳糖凝集素），并分析了它们的保守结构域、基序和共线性关系。（半乳糖凝集素）的表达受金黄色葡萄球菌和鳗弧菌感染的调控，表明其参与了对微生物病原体的免疫反应。对一个重要的成员 Galectin-3 进行了进一步分析，亚细胞定位显示 ToGal-3like 蛋白在细胞核和细胞质中均有表达。通过原核表达获得的重组蛋白表明，rToGal-3like 可以凝集兔、鲤鱼和金鲳的红细胞，还可以凝集和杀死金黄色葡萄球菌和鳗弧菌。本研究为进一步研究半乳糖凝集素在硬骨鱼中的免疫作用奠定了基础。
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