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一种用于蛋白激酶C活性细胞成像的高性能基因编码传感器。

A high-performance genetically encoded sensor for cellular imaging of PKC activity .

作者信息

Yahiro Takaki, Bayless-Edwards Landon, Jones James A, Ma Lei, Qin Maozhen, Mao Tianyi, Zhong Haining

机构信息

Vollum Institute, Oregon Health and Science University, Portland, OR, 97239.

出版信息

bioRxiv. 2024 Jul 23:2024.07.19.604387. doi: 10.1101/2024.07.19.604387.

DOI:10.1101/2024.07.19.604387
PMID:39091834
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11291028/
Abstract

We report a genetically encoded fluorescence lifetime sensor for protein kinase C (PKC) activity, named CKAR3, based on Förster resonance energy transfer. CKAR3 exhibits a 10-fold increased dynamic range compared to its parental sensors and enables imaging of PKC activity during animal behavior. Our results reveal robust PKC activity in a sparse neuronal subset in the motor cortex during locomotion, in part mediated by muscarinic acetylcholine receptors.

摘要

我们报告了一种基于Förster共振能量转移的用于蛋白激酶C（PKC）活性的基因编码荧光寿命传感器，名为CKAR3。与它的亲本传感器相比，CKAR3的动态范围增加了10倍，并能够在动物行为过程中对PKC活性进行成像。我们的结果揭示了运动过程中运动皮层中稀疏神经元亚群中强大的PKC活性，部分由毒蕈碱型乙酰胆碱受体介导。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/dfedc282b335/nihpp-2024.07.19.604387v1-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/bff6aa740766/nihpp-2024.07.19.604387v1-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/88640029cda7/nihpp-2024.07.19.604387v1-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/232af9b8793f/nihpp-2024.07.19.604387v1-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/0067286fdb34/nihpp-2024.07.19.604387v1-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/92c1e96ca08e/nihpp-2024.07.19.604387v1-f0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/db351e9cb412/nihpp-2024.07.19.604387v1-f0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/38ffe2b90c40/nihpp-2024.07.19.604387v1-f0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/2769a343305c/nihpp-2024.07.19.604387v1-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/2b8d0966bee8/nihpp-2024.07.19.604387v1-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/dfedc282b335/nihpp-2024.07.19.604387v1-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/bff6aa740766/nihpp-2024.07.19.604387v1-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/88640029cda7/nihpp-2024.07.19.604387v1-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/232af9b8793f/nihpp-2024.07.19.604387v1-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/0067286fdb34/nihpp-2024.07.19.604387v1-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/92c1e96ca08e/nihpp-2024.07.19.604387v1-f0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/db351e9cb412/nihpp-2024.07.19.604387v1-f0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/38ffe2b90c40/nihpp-2024.07.19.604387v1-f0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/2769a343305c/nihpp-2024.07.19.604387v1-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/2b8d0966bee8/nihpp-2024.07.19.604387v1-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6c4/11291028/dfedc282b335/nihpp-2024.07.19.604387v1-f0003.jpg

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本文引用的文献

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Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo.用于在体检测 cAMP 群体和亚细胞成像的敏感遗传编码传感器。
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Conventional protein kinase C in the brain: 40 years later.40年后的大脑中的传统蛋白激酶C
Neuronal Signal. 2017 Apr 10;1(2):NS20160005. doi: 10.1042/NS20160005. eCollection 2017 Apr.
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Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy.利用体内双光子荧光寿命成像显微镜观察头部固定行为小鼠中的蛋白激酶A活性
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