血清生物标志物转化生长因子-β和肿瘤坏死因子-α水平与糖尿病周围神经病变的关系。

Association of serum biomarkers level transforming growth factor-β and tumor necrosis factor-α with diabetic neuropathy.

机构信息

Department of Microbiology, College of Medicine, Al-Iraqia University, Baghdad, Iraq.

Department of Family and Community Medicine, College of Medicine, Al-Iraqia University, Baghdad, Iraq.

出版信息

Hum Antibodies. 2024;32(4):193-199. doi: 10.3233/HAB-240031.

DOI:10.3233/HAB-240031

PMID:39093067

Abstract

BACKGROUND

Many studies have examined the role of inflammation in the development of diabetic neuropathy (DPN).

OBJECTIVE

Evaluate the relation of the serum level of Transforming Growth Factor-β and Tumor Necrosis Factor-α and development of diabetic peripheral neuropathy DPN.

METHODS

In a case-control study, randomly selected 140 diabetic patients were included, the randomly selected patients were divided equally and matched into a case group who have diabetic peripheral neuropathy and diabetic neuropathy-free patients as a control group. For both groups whole blood sample was examined to compare for (TGF-β), and (TNF-α) levels determination by ELISA technique.

RESULTS

The age of the study samples ranged from 25 to 80 years with a male ratio of 1.45:1 although the sex differences between both groups were not significant. The mean levels of (TNF-α) and (TGF-β) was significantly higher among cases group than that of controls group (254.86 ± 75.9 vs158.01 ± 50.600) for TNF-α and for TGF- β (312.85 ± 62.27 vs. 217.82 ± 52.95) respectively. Both TNF-α and TGF-β have high sensitivity and specificity in detection of DPN. The sensitivity of TNF-α was 95.7% and specificity of 61.4% area under the ROC curve (AUC) of 0.870 ± 0.029, while the sensitivity of TGF-β was 91.4%, and specificity of 67.1 with good area under the ROC curve (AUC) of 0.891 ± 0.026 (P=0.000).

CONCLUSIONS

TNF-α and TGF -β are significantly elevated levels in patients with DPN, these cytokines could be used as indicators for the development of DPN.

摘要

背景

许多研究已经探讨了炎症在糖尿病周围神经病变（DPN）发展中的作用。

目的

评估转化生长因子-β和肿瘤坏死因子-α的血清水平与糖尿病周围神经病变（DPN）发展的关系。

方法

在一项病例对照研究中，随机选择了 140 名糖尿病患者，将随机选择的患者平均分为病例组和对照组，病例组为患有糖尿病周围神经病变的患者，对照组为无糖尿病周围神经病变的患者。对两组患者的全血样本进行检查，通过 ELISA 技术比较（TGF-β）和（TNF-α）水平的测定。

结果

研究样本的年龄范围为 25 至 80 岁，男性比例为 1.45:1，尽管两组之间的性别差异不显著。病例组的（TNF-α）和（TGF-β）平均水平明显高于对照组（254.86±75.9 对 158.01±50.600）TNF-α和 TGF-β 分别为（312.85±62.27 对 217.82±52.95）。TNF-α 和 TGF-β 对 DPN 的检测均具有较高的敏感性和特异性。TNF-α 的敏感性为 95.7%，特异性为 61.4%，ROC 曲线下面积（AUC）为 0.870±0.029，而 TGF-β 的敏感性为 91.4%，特异性为 67.1%，ROC 曲线下面积（AUC）为 0.891±0.026（P=0.000）。