Mushroom Research Centre, Universiti Malaya, Kuala Lumpur, Malaysia.
Department of Pharmaceutical Life Sciences, Faculty of Pharmacy, Universiti Malaya, 50603 Kuala Lumpur, Malaysia; Mushroom Research Centre, Universiti Malaya, 50603 Kuala Lumpur, Malaysia; Clinical Investigation Centre, 5th Floor, East Tower, University Malaya Medical Centre, 59100 Lembah Pantai Kuala Lumpur, Malaysia.
Int J Med Mushrooms. 2024;26(9):17-31. doi: 10.1615/IntJMedMushrooms.2024054164.
Cellular damage resulting from elevated levels of free radicals can lead to persistent health issues. Pleurotus floridanus, an edible white oyster mushroom, is rich in β-glucans with potent antioxidant and anti-inflammatory properties. In this research, we examined the β-glucan content, total phenolic content, as well as antioxidant and anti-inflammatory potential of hot water extracts with varying particle sizes (< 75, 75-154, 154-300, and 300-600 μm) of both whole and sliced fruiting bodies of P. floridanus. The findings revealed that the в-glucan content increased as the particle size increased, although no significant differences were observed. Conversely, smaller particle sizes (< 75 μm) of whole and sliced fruiting bodies of P. floridanus exhibited higher phenolic content, 2,2-diphenyl-1-picryl-hy-drazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging activity, and reducing ability compared with larger particle size (> 75 μm). Of the four samples (AW2, AW3, AS1, and AS2) with the highest antioxidant activity selected for anti-inflammatory assays, all demonstrated the ability to reduce nitric oxide and tumor necrosis factor-alpha levels, but did not enhance interleukin-10 expression in lipopolysaccharide-stimulated RAW264.7 cells. Interestingly, particle size < 75 to 300 μm did not appear to influence the anti-inflammatory activity, because no significant differences were observed among the particle sizes. Therefore, a particle size < 300 μm in a P. floridanus hot water extract could serve as a valuable source of antioxidant and anti-inflammatory compounds to counteract the harmful effects of free radicals.
细胞损伤是由自由基水平升高引起的，可能会导致持续的健康问题。佛罗里达侧耳，一种可食用的白蚝菇，富含具有强大抗氧化和抗炎特性的β-葡聚糖。在这项研究中，我们研究了不同粒径 (< 75、75-154、154-300 和 300-600 μm) 的整菇和切片的佛罗里达侧耳热水提取物的β-葡聚糖含量、总酚含量以及抗氧化和抗炎潜力。研究结果表明，β-葡聚糖含量随粒径的增加而增加，尽管没有显著差异。相反，整菇和切片的佛罗里达侧耳的较小粒径 (< 75 μm) 表现出更高的酚类含量、2,2-二苯基-1-苦基肼 (DPPH) 和 2,2'-联氮-双(3-乙基苯并噻唑啉-6-磺酸) (ABTS) 清除活性和还原能力，与较大粒径 (> 75 μm) 相比。在四种具有最高抗氧化活性的样品 (AW2、AW3、AS1 和 AS2) 中选择用于抗炎测定，所有样品均显示出降低一氧化氮和肿瘤坏死因子-α水平的能力，但在脂多糖刺激的 RAW264.7 细胞中并未增强白细胞介素-10 的表达。有趣的是，粒径 < 75 至 300 μm 似乎不会影响抗炎活性，因为粒径之间没有观察到显著差异。因此，粒径 < 300 μm 的佛罗里达侧耳热水提取物可以作为抗氧化和抗炎化合物的有价值来源，以抵消自由基的有害影响。
