Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China.
Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China.
Forensic Sci Int. 2024 Sep;362:112148. doi: 10.1016/j.forsciint.2024.112148. Epub 2024 Jul 14.
Recently, RNA markers have been used to identify tissue origins of different kinds of body fluids. Herein, circRNA and miRNA markers were carried out to examine the presence or absence of peripheral blood (PB) in bloodstained samples exposed to different external environmental conditions, which mimicked PB samples left at the crime scenes. PB samples were placed on sterile swabs and then exposed to different high temperatures (37°C, 55°C and 95°C) and ultraviolet light irradiation for 0 d, 0.5 d, 1 d, 3 d, and 7 d, ultra-low and low temperatures (-80°C, -20°C, and 4°C) for 30 d, 180 d and 365 d and different kinds of disinfectants. Total RNA was extracted from bloodstained samples under the above different conditions, and the expressions of target RNAs (including miR16-5p, miR451a, circ0000095, and two reference genes RNU6b and 18 S rRNA) were detected by the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method. Results showed that these selected RNA markers could be successfully measured at all observation points with their unique degradation rates, which exhibited relative stability in degraded bloodstained samples exposed to different environmental conditions. This study provides insights into the applications of these studied miRNA and circRNA markers in forensic science.
最近，RNA 标志物已被用于鉴定不同类型体液的组织来源。在此，通过circRNA 和 miRNA 标志物检测，研究了在模拟犯罪现场遗留的外周血 (PB) 样本暴露于不同外部环境条件下是否存在 PB。将 PB 样本放置在无菌拭子上，然后分别暴露于不同高温 (37°C、55°C 和 95°C) 和紫外线照射 0 d、0.5 d、1 d、3 d 和 7 d，超低温和低温 (-80°C、-20°C 和 4°C) 30 d、180 d 和 365 d 以及不同种类的消毒剂下。从上述不同条件下的血斑样本中提取总 RNA，并通过逆转录-定量聚合酶链反应 (RT-qPCR) 方法检测目标 RNA（包括 miR16-5p、miR451a、circ0000095 和两个参考基因 RNU6b 和 18S rRNA）的表达。结果表明，这些选定的 RNA 标志物可以在所有观察点以其独特的降解率成功测量，并且在暴露于不同环境条件下的降解血斑样本中表现出相对稳定性。本研究为这些研究中的 miRNA 和 circRNA 标志物在法医学中的应用提供了新的见解。
