Department of Dental Anthropology, Chair of Forensic dentistry, University of Zagreb School of Dental Medicine, Zagreb, Croatia.
Department of Orthodontics, University of Zagreb School of Dental Medicine, Zagreb, Croatia.
Int J Legal Med. 2024 Nov;138(6):2459-2467. doi: 10.1007/s00414-024-03297-x. Epub 2024 Aug 3.
Our research was aimed to establish the equation and to determine its precision for dental age estimation in adolescents, adults and the elderly based on the thickness of deposited dental cement.
The study sample consisted of 206 donor teeth of both sexes (10 to 82 years of age). The roots were transversely cut (6 cuts) on an ISOMET 1000 cutter at apical, middle, and cervical parts (slice thickness 0.3 to 0.5 μm). Measurements of cement thickness on incisions were made with a light microscope and an Olympus EP50 camera with previous microscope calibration for each measurement episode. Measurements were carried out clockwise at 4 measuring points on each incision. Statistical analyses were conducted using statistical software packages STATISTICA version 12.0 StatSoft, Inc. 2013, and MedCalc version 22.0.
Mean cement thickness (MCT) significantly declined from apical to cervical cuts (P < 0.001, ANOVA) and was thicker in teeth with a destroyed tooth crown (P < 0.001, ANOVA) and in molars compared to incisors (P < 0.001, ANOVA). MCT was greater in men and in teeth with more than one root but without reaching statistical significance. MCT showed a significant increase from the second decade of chronological age with a peek at sixth decade and declined to the ninth decade (P < 0.001, ANOVA). Piecewise linear regression defined a breakpoint age of 53 years, so two different equations were calculated (≤ 53 years and > 53 years).
The resulting equation is recommended for estimating dental age in forensic analyses.
本研究旨在建立并确定基于牙骨质沉积厚度的青少年、成人和老年人牙龄估算方程及其精度。
研究样本由 206 颗来自男女供体的牙齿组成（年龄 10 至 82 岁）。将根尖、中部和颈部的牙根部（切片厚度 0.3 至 0.5 μm）进行横向切割（6 刀）。在 ISOMET 1000 切割器上使用 Olympus EP50 相机和显微镜对每个测量片段进行校准后，在切面上用显微镜测量牙骨质的厚度。测量在每个切口上沿顺时针方向在 4 个测量点进行。使用 STATISTICA 版本 12.0 StatSoft，Inc. 2013 和 MedCalc 版本 22.0 统计软件包进行统计分析。
牙骨质厚度（MCT）从根尖向颈部逐渐减少（P < 0.001，方差分析），牙冠破坏的牙齿（P < 0.001，方差分析）和磨牙的 MCT 较切牙厚（P < 0.001，方差分析）。男性和具有多于一个根但无统计学意义的牙齿的 MCT 较大。MCT 从第二个十年的实际年龄开始显著增加，在第六个十年达到峰值，然后下降到第九个十年（P < 0.001，方差分析）。分段线性回归定义了 53 岁的年龄断点，因此计算了两个不同的方程（≤ 53 岁和> 53 岁）。
推荐使用该方程进行法医分析中的牙龄估计。
