Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, P. R. China.
National Engineering Research Center of Solid-State Brewing, Luzhou Laojiao Group Co. Ltd., Luzhou, 646000, P. R. China.
Small. 2024 Nov;20(46):e2402674. doi: 10.1002/smll.202402674. Epub 2024 Aug 3.
Hydrolytic enzymes are essential components in second-generation biofuel technology and food fermentation processes. Nanozymes show promise for large-scale industrial applications as replacements for natural enzymes due to their distinct advantages. However, there remains a research gap concerning glycosidase nanozymes. In this study, a Zn-based single-atom nanozyme (ZnN-900) is developed for efficient glycosidic bond hydrolysis in an aqueous solution. The planar structure of the class-porphyrin N material approximatively mimicked the catalytic centers of natural enzymes, facilitating oxidase-like (OXD-like) activity and promoting glycosidic bond cleavage. Theoretical calculations show that the Zn site can act as Lewis acids, attacking the C─O bond in glycosidic bonds. Additionally, ZnN-900 has the ability to degrade starch and produce reducing sugars that increased yeast cell biomass by 32.86% and ethanol production by 14.56%. This catalyst held promising potential for enhancing processes in ethanol brewing and starch degradation industries.
水解酶是第二代生物燃料技术和食品发酵过程中的重要组成部分。纳米酶由于其独特的优势，有望大规模应用于工业领域，替代天然酶。然而，糖苷酶纳米酶的研究仍存在空白。本研究开发了一种基于锌的单原子纳米酶（ZnN-900），用于在水溶液中高效水解糖苷键。类卟啉 N 材料的平面结构近似模拟了天然酶的催化中心，促进了氧化酶样（OXD-like）活性并促进糖苷键断裂。理论计算表明，Zn 位可以充当路易斯酸，攻击糖苷键中的 C─O 键。此外，ZnN-900 能够降解淀粉并产生还原糖，使酵母细胞生物量增加 32.86%，乙醇产量增加 14.56%。该催化剂有望提高乙醇酿造和淀粉降解行业的工艺水平。
