College of Environment and Climate, Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China.
China CDC Key Laboratory of Environment and Public Health, National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
Environ Sci Technol. 2024 Aug 13;58(32):14088-14097. doi: 10.1021/acs.est.4c01146. Epub 2024 Aug 3.
Urinary analysis of exogenous and endogenous molecules constitutes an efficient, noninvasive approach to evaluate human health status. However, the exposome characterization of urinary molecules remains extremely challenging with current techniques. Herein, we develop an ExpoNano strategy based on hyper-cross-linked polymers (HCPs) to achieve ultrahigh-throughput measurement of exo/endogenous molecules in urine. The strategy includes a simple trapping-detrapping procedure (15 min) with HCPs in enzymatically treated urine, followed by mass spectrometer determination. Molecules that can be determined by ExpoNano have a wide range of molecular weight (75-837 Da) and Log (octanol-water partition coefficient; -9.86 to 10.56). The HCPs can be repeatedly used five times without decreasing the trapping efficiency. Application of ExpoNano in a biomonitoring study revealed a total of 63 environmental chemicals detected in >50% of the urine pools collected from Chinese adults living in 13 cities, with a median concentration of 0.026-47 ng/mL, while nontargeted analysis detected an additional 243 exogenous molecules. Targeted and nontargeted analysis also detected 926 endogenous molecules in pooled urine. Collectively, the ExpoNano strategy demonstrates unique advantages over traditional urine analysis approaches, including a wide range of analytes, satisfactory trapping efficiency, high simplicity and reusability, and extremely reduced time demand and financial cost.
外源性和内源性分子的尿液分析是评估人体健康状况的一种有效、非侵入性的方法。然而，利用现有技术对外泌体分子进行特征描述仍然极具挑战性。在此，我们开发了一种基于超交联聚合物（HCPs）的 ExpoNano 策略，以实现尿液中外源/内源性分子的超高通量测量。该策略包括在经酶处理的尿液中用 HCPs 进行简单的捕获-解吸程序（15 分钟），然后用质谱仪进行测定。可以用 ExpoNano 测定的分子具有广泛的分子量（75-837 Da）和 Log （辛醇-水分配系数；-9.86 至 10.56）。HCPs 可以重复使用五次而不会降低捕获效率。ExpoNano 在一项生物监测研究中的应用显示，从中国 13 个城市的成年人收集的尿液样本中，共有 63 种环境化学物质在>50%的尿液样本中被检测到，中位数浓度为 0.026-47ng/mL，而非靶向分析则检测到另外 243 种外源性分子。靶向和非靶向分析还在混合尿液中检测到 926 种内源性分子。总的来说，ExpoNano 策略与传统的尿液分析方法相比具有独特的优势，包括广泛的分析物、令人满意的捕获效率、高的简单性和可重复性，以及极低的时间需求和经济成本。
