Department of Physics, University of Pretoria, Pretoria, Gauteng, South Africa.
Department of Physics, University of Pretoria, Pretoria, Gauteng, South Africa; Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, Gauteng, South Africa; National Institute of Theoretical and Computational Sciences (NITheCS), South Africa.
Biophys Rep (N Y). 2024 Sep 11;4(3):100173. doi: 10.1016/j.bpr.2024.100173. Epub 2024 Aug 2.
We present Full SMS, a multipurpose graphical user interface (GUI)-based software package for analyzing single-molecule spectroscopy (SMS) data. SMS typically delivers multiparameter data-such as fluorescence brightness, lifetime, and spectra-of molecular- or nanometer-scale particles such as single dye molecules, quantum dots, or fluorescently labeled biological macromolecules. Full SMS allows an unbiased statistical analysis of fluorescence brightness through level resolution and clustering, analysis of fluorescence lifetimes through decay fitting, as well as the calculation of second-order correlation functions and the display of fluorescence spectra and raster-scan images. Additional features include extensive data filtering options, a custom HDF5-based file format, and flexible data export options. The software is open source and written in Python but GUI based so it may be used without any programming knowledge. A multiprocess architecture was employed for computational efficiency. The software is also designed to be easily extendable to include additional import data types and analysis capabilities.
我们介绍了 Full SMS，这是一个基于图形用户界面 (GUI) 的多用途软件包，用于分析单分子光谱 (SMS) 数据。SMS 通常提供多参数数据，例如分子或纳米级颗粒（如单个染料分子、量子点或荧光标记的生物大分子）的荧光亮度、寿命和光谱。Full SMS 允许通过电平分辨率和聚类对荧光亮度进行无偏统计分析，通过衰减拟合对荧光寿命进行分析，以及计算二阶相关函数和显示荧光光谱和光栅扫描图像。其他功能包括广泛的数据过滤选项、基于自定义 HDF5 的文件格式以及灵活的数据导出选项。该软件是开源的，用 Python 编写，但基于 GUI，因此无需任何编程知识即可使用。采用了多进程架构以提高计算效率。该软件还设计为易于扩展，以包括其他导入数据类型和分析功能。
