Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695-7905, USA.
Bioresour Technol. 2024 Sep;408:131198. doi: 10.1016/j.biortech.2024.131198. Epub 2024 Aug 6.
Factors that contribute to optimal chalcopyrite bioleaching by extremely thermoacidophilic archaea were examined for ten species belonging to the order Sulfolobales from the genera Acidianus (A. brierleyi), Metallosphaera (M. hakonensis, M. sedula, M. prunae), Sulfuracidifex (S. metallicus, S. tepriarius), Sulfolobus (S. acidocaldarius), Saccharlobus (S. solfataricus) and Sulfurisphaera (S. ohwakuensis, S. tokodaii). Only A. brierleyi, M. sedula, S. metallicus, S. tepriarius, S. ohwakuensis, and S. tokodai exhibited significant amounts of bioleaching and were investigated further. At 70-75 °C, Chalcopyrite loadings of 10 g/l were leached for 21 days during which pH, redox potential, planktonic cell density, iron concentrations and sulfate levels were monitored, in addition to copper mobilization. S. ohwakuensis proved to be the most prolific bioleacher. This was attributed to balanced iron and sulfur oxidation, thereby reducing by-product (e.g., jarosites) formation and minimizing surface passivation. Comparative genomics suggest markers for bioleaching potential, but the results here point to the need for experimental verification.
考察了 10 种属于硫单胞菌目（Acidianus（A. brierleyi）、Metallosphaera（M. hakonensis、M. sedula、M. prunae）、Sulfuracidifex（S. metallicus、S. tepriarius）、Sulfolobus（S. acidocaldarius）、Saccharlobus（S. solfataricus）和 Sulfurisphaera（S. ohwakuensis、S. tokodaii））的极端嗜热嗜酸古菌对黄铜矿生物浸出的最佳因素。只有 A. brierleyi、M. sedula、S. metallicus、S. tepriarius、S. ohwakuensis 和 S. tokodai 表现出显著的生物浸出能力，并进一步进行了研究。在 70-75°C 下，用 10g/l 的黄铜矿负载量进行了 21 天的浸出，在此期间监测了 pH 值、氧化还原电位、浮游细胞密度、铁浓度和硫酸盐水平，以及铜的释放。S. ohwakuensis 被证明是最有效的生物浸出菌。这归因于铁和硫的氧化平衡，从而减少了副产物（如 jarosites）的形成，并最小化了表面钝化。比较基因组学为生物浸出潜力提供了标记，但这里的结果表明需要进行实验验证。
