Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.
Research and Development Laboratory, Sanwa Shurui Co., Ltd., 2231-1 Yamamoto, Usa, Oita, 879-0495, Japan.
Anal Sci. 2024 Nov;40(11):2043-2051. doi: 10.1007/s44211-024-00641-4. Epub 2024 Aug 3.
Saccharomyces cerevisiae, a widely studied yeast known for its industrial applications, is increasingly recognized for its potential in immunomodulation. This study aimed to systematically analyze and compare the immune-modulating properties of various S. cerevisiae strains under controlled experimental conditions. Three essential signals crucial for immune response activation were evaluated to elucidate the immunological responses elicited by these strains, i.e., dendritic cells (DC) cytokine secretion profiles, maturation status, and T cell polarization. Analysis of DC cytokine secretion profiles and maturation status revealed that all tested yeast strains induced DC activation, characterized by significant IL-6 secretion and modest IL-10 induction, as well as upregulation of MHC II molecules. Additionally, strain-specific effects were observed, particularly, strain AJM109 and Y1383 uniquely enhanced CD86 and PD-L1 expression, respectively, suggesting differential impacts on DC co-stimulatory signaling. Furthermore, strain Y1383 showed a unique capacity to support Treg-mediated immune suppression, demonstrating its potential in immune tolerance induction. These findings underscore the complexity of S. cerevisiae-based immune modulation and emphasize the importance of standardized evaluation methods to distinguish their specific immunological effects.
酿酒酵母是一种广泛研究的酵母，因其在工业应用中的作用而备受关注，同时也逐渐被认识到其在免疫调节方面的潜力。本研究旨在系统分析和比较在受控实验条件下各种酿酒酵母菌株的免疫调节特性。评估了三个对免疫反应激活至关重要的信号，以阐明这些菌株引起的免疫反应，即树突状细胞（DC）细胞因子分泌谱、成熟状态和 T 细胞极化。对 DC 细胞因子分泌谱和成熟状态的分析表明，所有测试的酵母菌株均诱导了 DC 激活，其特征为显著的 IL-6 分泌和适度的 IL-10 诱导，以及 MHC II 分子的上调。此外，还观察到菌株特异性效应，特别是菌株 AJM109 和 Y1383 分别独特地增强了 CD86 和 PD-L1 的表达，表明对 DC 共刺激信号有不同的影响。此外，菌株 Y1383 显示出独特的支持 Treg 介导的免疫抑制能力，表明其在诱导免疫耐受方面的潜力。这些发现强调了基于酿酒酵母的免疫调节的复杂性，并强调了采用标准化评估方法来区分其特定免疫效应的重要性。
