Department of Gastroenterology, The Affiliated Lianyungang Hospital of Xuzhou Medical University/The First People's Hospital of Lianyungang, Lianyungang, China.
Cell Mol Biol (Noisy-le-grand). 2024 Jul 28;70(7):206-211. doi: 10.14715/cmb/2024.70.7.30.
The objective of this study was to investigate the impact of ethyl pyruvate (EP), an HMGB1 inhibitor, on ESCC cells both in vitro and in vivo. The viability of ESCC cells was assessed using the MTT method to evaluate the correlation between EP and cell viability. A scratch test was used to investigate the relationship between EP and cell migration and invasion. The effects of EP on tumor growth and survival in cancerous nude mice were examined using a tumor formation model. Immunohistochemical staining was performed to evaluate the expression levels of HMGB1, TLR4, and MyD88 in tumor tissues. EP, an anti-HMGB1 inhibitor, inhibited ESCC cell proliferation and metastasis in vitro and in vivo. Furthermore, compared with the control treatment, EP improved the activity, diet, and drinking behaviour of nude mice; inhibited tumour growth; and led to lower protein expression levels of HMGB1, TLR4, and MyD88. EP has the potential to regulate the HMGB1/TLR4-MyD88 signaling pathway, thereby inhibiting the proliferation and metastasis of ESCC, suppressing tumor growth, improving quality of life, and serving as an effective drug for ESCC treatment.
本研究旨在探讨 1-乙基-2-吡咯烷酮（EP），一种高迁移率族蛋白 1（HMGB1）抑制剂，对 ESCC 细胞的体内外作用。采用 MTT 法评估 ESCC 细胞活力，以评估 EP 与细胞活力的相关性。划痕实验用于研究 EP 与细胞迁移和侵袭的关系。肿瘤形成模型用于研究 EP 对荷瘤裸鼠肿瘤生长和生存的影响。免疫组化染色用于评估肿瘤组织中 HMGB1、TLR4 和 MyD88 的表达水平。EP 作为抗 HMGB1 抑制剂，可抑制 ESCC 细胞的增殖和转移，无论是在体外还是体内。此外，与对照组相比，EP 改善了裸鼠的活动、饮食和饮水行为；抑制了肿瘤生长；并导致 HMGB1、TLR4 和 MyD88 的蛋白表达水平降低。EP 可能通过调节 HMGB1/TLR4-MyD88 信号通路，从而抑制 ESCC 的增殖和转移，抑制肿瘤生长，提高生活质量，成为 ESCC 治疗的有效药物。
