Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Jiading Branch of Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 800 Huangjiahuayuan Road, Shanghai 201803, P.R.China.
Cell Mol Biol (Noisy-le-grand). 2024 Jul 28;70(7):22-28. doi: 10.14715/cmb/2024.70.7.4.
Vitrification has important application in assisted reproductive technology (ART) and this technique has been widely used in the cryopreservation of oocytes and embryos. However, due to susceptibility of epigenetic modifications to environmental changes induced by cryopreservation procedures, there are concerns about the potential epigenetic consequences of oocyte and embryo vitrification. This review comprehensively summarized the effect of cryopreservation-especially the vitrification method in ART-on oocytes and embryos. Various studies have reported changes in different aspects of genomic status which directly affect the quality of fertilized embryos. The objective of this review is to assess existing literature on the epigenetic modifications that occur in vitrified oocytes and early embryos resulting from oocyte vitrification, including DNA modifications, RNA methylation, histone modification and microRNAs related to ART.
玻璃化在辅助生殖技术（ART）中具有重要的应用，这项技术已广泛应用于卵母细胞和胚胎的冷冻保存。然而，由于胚胎的表观遗传修饰易受冷冻保存过程中环境变化的影响，人们对卵母细胞和胚胎玻璃化的潜在表观遗传后果表示担忧。本文全面总结了冷冻保存（尤其是 ART 中的玻璃化方法）对卵母细胞和胚胎的影响。各种研究报告称，基因组状态的不同方面发生了变化，这直接影响了受精卵胚胎的质量。本综述的目的是评估现有的关于卵母细胞玻璃化导致的卵母细胞和早期胚胎中发生的表观遗传修饰的文献，包括 DNA 修饰、RNA 甲基化、组蛋白修饰和与 ART 相关的 microRNAs。
