Sun Yuxiang, Cao Ziqi, Zhang Xiaoli, Zhu Xingchen, Xu Zhenyang, Zhou Hantong, Wei Xiaoer, Du Wenxian, Xu Li
Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou, 225001, P. R. China.
Department of Periodontology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, 210008, P. R. China.
Small Methods. 2025 Mar;9(3):e2400945. doi: 10.1002/smtd.202400945. Epub 2024 Aug 4.
Tumor photodynamic therapy (PDT) relies on intratumoral free radicals, while the limited oxygen source and the depletion of tissue oxygen may exacerbate the hypoxia. As the treatment progresses, there will eventually be a problem of insufficient free radicals. Here, it is found that Au@CeO nano-rods (Au@Ce NRs), assembled by gold nano-rods (Au NRs) and ceria nanoparticles (CeO NPs), can efficaciously absorb near-infrared light (NIR) to promote the release of oxygen and free radicals. Au@Ce NRs exhibit a higher proportion of Ce (CeO) after oxygen release, while Ce is subsequently oxidized to Ce (CeO) by trace HO. Interestingly, Au@Ce NRs re-oxidized by trace HO can re-release oxygen and free radicals again upon NIR treatment, achieving oxygenation/oxygen evolution, similar to charging/discharging. This loop maximizes the conversion of limited oxygen source into highly cytotoxic free radicals. As a result, when B16-F10 cells are treated by NIR/Au@Ce NRs, more tumor cells undergo apoptosis, consistent with the higher level of free radicals. Importantly, NIR/Au@Ce NRs successfully suppresses tumor growth and promotes the generation of epidermal collagen fibers in the transplanted tumor model. Therefore, the rod-shaped Au@Ce NRs provide an ideal platform for maximizing the utilization of intratumoral oxygen sources and improving the treatment of melanoma.
肿瘤光动力疗法（PDT）依赖于肿瘤内的自由基，而有限的氧源和组织氧的消耗可能会加剧缺氧。随着治疗的进行，最终会出现自由基不足的问题。在此，发现由金纳米棒（Au NRs）和二氧化铈纳米颗粒（CeO NPs）组装而成的Au@CeO纳米棒（Au@Ce NRs）能够有效吸收近红外光（NIR）以促进氧气和自由基的释放。Au@Ce NRs在释放氧气后表现出更高比例的Ce（CeO），而Ce随后会被微量的HO氧化为Ce（CeO）。有趣的是，被微量HO再氧化的Au@Ce NRs在近红外处理后能够再次释放氧气和自由基，实现氧合/氧气释放，类似于充电/放电。这个循环将有限的氧源最大限度地转化为具有高细胞毒性的自由基。结果，当用近红外光/NIR/Au@Ce NRs处理B16-F10细胞时，更多的肿瘤细胞发生凋亡，这与更高水平的自由基一致。重要的是，近红外光/NIR/Au@Ce NRs在移植瘤模型中成功抑制了肿瘤生长并促进了表皮胶原纤维的生成。因此，棒状的Au@Ce NRs为最大限度地利用肿瘤内氧源和改善黑色素瘤治疗提供了一个理想的平台。
