State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Textile Industrial Products Testing Center of Nanjing Customs District, Wuxi Customs District PR China, Wuxi, 214100, China.
Talanta. 2024 Nov 1;279:126653. doi: 10.1016/j.talanta.2024.126653. Epub 2024 Jul 31.
Patulin (PAT) is a mycotoxin-produced secondary metabolite that can contaminate foods, causing toxic effects on animal and human health. Therefore, for the first time, we have constructed a "turn-on" dual-mode aptamer sensor for PAT using oleic acid-coated upconversion nanomaterials (OA-UCNPs) and G-Quadruplex-hemin DNAzyme (G4-DNAzyme) as fluorescent and colorimetry probes. The sensor employs aptamers binding to PAT as recognition elements for specific molecule detection. Mxene-Au can be used as a biological inducer to assist OA-UCNPs in controlling fluorescence intensity. In addition, colorimetric signal amplification was performed using the trivalent G4-DNAzyme to increase detection sensitivity and reduce false positives. Under optimal conditions, the dual-mode aptasensor has a detection limit of 5.3 pg mL in fluorescence and 2.4 pg mL in colorimetric methods, respectively, with the wider linear range and limit of detection (LOD) of the colorimetric assay. The combination aptasensor can detect PAT with high sensitivity and high specificity and has broad application prospects in the field of food safety detection.
棒曲霉素（PAT）是一种由真菌产生的次级代谢产物，可污染食品，对动物和人类健康造成毒性影响。因此，我们首次构建了一种基于油酸包覆上转换纳米材料（OA-UCNPs）和 G-四链体-血红素 DNA 酶（G4-DNAzyme）的“开启”双模式适配体传感器，用作荧光和比色探针用于 PAT。该传感器采用适配体作为识别元件与 PAT 结合，用于特定分子的检测。Mxene-Au 可用作生物诱导剂，辅助 OA-UCNPs 控制荧光强度。此外，使用三价 G4-DNA 酶进行比色信号放大，以提高检测灵敏度并减少假阳性。在最佳条件下，双模式适配体传感器在荧光检测中的检出限为 5.3pgmL，在比色检测中的检出限为 2.4pgmL，比色法具有更宽的线性范围和检出限（LOD）。组合适配体传感器具有高灵敏度和高特异性，在食品安全检测领域具有广阔的应用前景。
