School of Resource and Environmental Sciences, Hubei Key Laboratory of Biomass-Resources Chemistry and Environmental Biotechnology, Hubei Research Center of Environment Remediation Technology, Wuhan University, Wuhan 430079, China.
School of Resource and Environmental Sciences, Hubei Key Laboratory of Biomass-Resources Chemistry and Environmental Biotechnology, Hubei Research Center of Environment Remediation Technology, Wuhan University, Wuhan 430079, China.
Sci Total Environ. 2024 Nov 1;949:175215. doi: 10.1016/j.scitotenv.2024.175215. Epub 2024 Aug 2.
Both soluble phosphorus (P) deficiency and petroleum hydrocarbon contamination represent challenges in soil environments. While phosphate-solubilizing bacteria and hydrocarbon-degrading bacteria have been identified and employed in environmental bioremediation, the bacteria co-adapted to soluble P deficiency and hydrocarbon contamination has rarely been reported. This study explored the ability of Acinetobacter oleivorans S4 (A. oleivorans S4) to solubilize phosphate using n-hexadecane (H), glucose (G), and a mixed carbon source (HG) in tricalcium phosphate (TCP) medium. A. oleivorans S4 exhibited robust growth in H-TCP, releasing 31 mg L of soluble P. Conversely, A. oleivorans S4 barely grew in G-TCP, releasing 654 mg L of soluble P. In HG-TCP, biomass surpassed that in H-TCP, with phosphate release comparable to that in G-TCP. HPLC analysis revealed a small amount of TCA cycle acids in H-TCP and a large amount of gluconate in G-TCP and HG-TCP. Transcriptomic analysis showed elevated expression of genes associated with alkane degradation, P starvation, N utilization, and trehalose synthesis in H-TCP, revealing the molecular co-adaptation mechanism of A. oleivorans S4. Furthermore, the addition of glucose enhanced alkane degradation, P and N utilization, and reduced trehalose synthesis. It indicated that incomplete glucose metabolism may provide energy for other reactions, and the increase in soluble P mediated by gluconate may alleviate oxidative stress. Overall, A. oleivorans S4 proves promising for remediating soluble P-deficient and hydrocarbon-contaminated environments, and glucose stimulates its transformation into a super phosphate-solubilizing bacterium.
可溶解磷（P）缺乏和石油烃污染均对土壤环境构成挑战。虽然已经鉴定出并在环境生物修复中使用了磷酸溶解细菌和烃降解细菌，但很少有报道表明细菌适应可溶解 P 缺乏和烃污染。本研究探索了不动杆菌 S4（A. oleivorans S4）在磷酸三钙（TCP）培养基中使用正十六烷（H）、葡萄糖（G）和混合碳源（HG）溶解磷的能力。A. oleivorans S4 在 H-TCP 中表现出强大的生长能力，释放出 31mg/L 的可溶解磷。相反，A. oleivorans S4 在 G-TCP 中几乎无法生长，释放出 654mg/L 的可溶解磷。在 HG-TCP 中，生物量超过了 H-TCP，磷酸盐释放量与 G-TCP 相当。HPLC 分析表明，在 H-TCP 中存在少量三羧酸循环酸，在 G-TCP 和 HG-TCP 中存在大量葡萄糖酸盐。转录组分析表明，在 H-TCP 中与烷烃降解、P 饥饿、N 利用和海藻糖合成相关的基因表达上调，揭示了 A. oleivorans S4 的分子协同适应机制。此外，葡萄糖的添加增强了烷烃降解、P 和 N 利用，并减少了海藻糖的合成。这表明不完全的葡萄糖代谢可能为其他反应提供能量，而葡萄糖酸盐介导的可溶解 P 增加可能减轻氧化应激。总体而言，A. oleivorans S4 有望用于修复可溶解 P 缺乏和烃污染的环境，而葡萄糖刺激其转化为超级磷酸溶解细菌。
