Korshunova Tatyana, Kuzina Elena, Mukhamatdyarova Svetlana, Iskuzhina Milyausha, Kulbaeva Liliya, Petrova Svetlana
Ufa Institute of Biology, Ufa Federal Research Centre, Russian Academy of Sciences, Ufa 450054, Russia.
Ufa Institute of Chemistry, Ufa Federal Research Centre, Russian Academy of Sciences, Ufa 450054, Russia.
Plants (Basel). 2024 Dec 20;13(24):3560. doi: 10.3390/plants13243560.
Biological remediation of agricultural soils contaminated with oil is complicated by the presence of residual amounts of chemical plant protection products, in particular, herbicides, which, like oil, negatively affect the soil microbiome and plants. In this work, we studied five strains of bacteria of the genera and , which exhibited a high degree of oil biodegradation (72-96%). All strains showed resistance to herbicides based on 2,4-D, imazethapyr and tribenuron-methyl, the ability to fix nitrogen, phosphate mobilization, and production of indole-3-acetic acid. The presence of pollutants affected the growth-stimulating properties of bacteria in different ways. The most promising strain N2 was used alone and together with oat and lupine plants for soil remediation of oil, including herbicide-treated oil-contaminated soil. Combined contamination was more toxic to plants and soil microorganisms. Bacterization stimulated the formation of chlorophyll and suppressed the synthesis of abscisic acid and malonic dialdehyde in plant tissues. The combined use of bacteria and oat plants most effectively reduced the content of hydrocarbons in the soil (including in the presence of herbicides). The results obtained can be used to develop new methods for bioremediation of soils with polychemical pollution.
受石油污染的农业土壤的生物修复因残留的化学植物保护产品，特别是除草剂的存在而变得复杂，这些除草剂与石油一样，会对土壤微生物群落和植物产生负面影响。在这项工作中，我们研究了 属和 属的五株细菌，它们表现出高度的石油生物降解能力（72-96%）。所有菌株都对基于 2,4-滴、咪唑乙烟酸和苯磺隆的除草剂具有抗性，具有固氮能力、磷素活化能力以及吲哚-3-乙酸的产生能力。污染物的存在以不同方式影响细菌的生长刺激特性。最有前景的菌株N2单独使用以及与燕麦和羽扇豆植物一起用于石油污染土壤的修复，包括经过除草剂处理的石油污染土壤。复合污染对植物和土壤微生物的毒性更大。细菌接种刺激了植物组织中叶绿素的形成，并抑制了脱落酸和丙二醛的合成。细菌与燕麦植物的联合使用最有效地降低了土壤中的碳氢化合物含量（包括在存在除草剂的情况下）。所获得的结果可用于开发多化学污染土壤生物修复的新方法。
