微血管密度与肿瘤相关巨噬细胞与口腔鳞状细胞癌进展的关系。

Relationship of microvascular density and tumor-associated macrophages with orofacial squamous cell carcinoma progression.

机构信息

Department of Oral Pathology, Fatima Memorial Hospital College of Medicine and Dentistry, University of Health Sciences, Lahore, Pakistan.

Department of Oral Biology and Tooth Morphology, Islamic International Dental College, Riphah International University, Islamabad, Pakistan; Department of Health Sciences, The Equator University of Science and Technology, Uganda.

出版信息

Oral Surg Oral Med Oral Pathol Oral Radiol. 2024 Oct;138(4):532-542. doi: 10.1016/j.oooo.2024.06.013. Epub 2024 Jun 24.

DOI:10.1016/j.oooo.2024.06.013

PMID:39098442

Abstract

OBJECTIVE

The current study aimed to investigate the characteristics of tumor-associated macrophages (TAMs) and their association with microvascular density (MVD) in tumor progression in different grades of orofacial squamous cell carcinoma (OSCC) in the Pakistani population.

STUDY DESIGN

This prospective study included 234 patients with oral cancer reported at different hospitals in Pakistan diagnosed with OSCC. Tumors were graded on the Anneroth grading system and the association between the frequency of TAMs and MVD was examined in vivo. The macrophages visible through immunohistochemistry for CD68 and the microvessels observed through immunohistochemistry for CD34 were manually counted in 3 high-power fields.

RESULTS

The CD68 and CD34 counts were significantly lower in well-differentiated squamous cell carcinoma compared to poorly differentiated squamous cell carcinoma. Linear regression analysis revealed a positive correlation between the area percentage of CD68 immunoreactivity and the grade of the tumor (r = 0.776). Vice versa, a positive correlation also existed between the area percentage of CD34 immunoreactivity and the grade of the tumor (r = 0.690). Pearson correlation revealed a positive association between the TAMs and MVD (r = 0.680; P < .001).

CONCLUSIONS

There was an increased population of tumor-associated macrophages and tumor angiogenesis with the increasing grade of orofacial squamous cell carcinoma. (Oral Surg Oral Med Oral Pathol Oral Radiol YEAR;VOL:page range).

摘要

目的

本研究旨在探讨巴基斯坦人口中不同分级口腔鳞状细胞癌（OSCC）中肿瘤相关巨噬细胞（TAMs）的特征及其与微血管密度（MVD）的关系。

研究设计

本前瞻性研究纳入了在巴基斯坦不同医院报告的 234 例口腔癌患者，这些患者被诊断为 OSCC。根据 Anneroth 分级系统对肿瘤进行分级，并在体内检查 TAMs 频率与 MVD 的相关性。通过 CD68 的免疫组织化学可见巨噬细胞，通过 CD34 的免疫组织化学可见微血管，在 3 个高倍视野中手动计数。

结果

与低分化鳞状细胞癌相比，高分化鳞状细胞癌中 CD68 和 CD34 的计数明显较低。线性回归分析显示，CD68 免疫反应性的面积百分比与肿瘤分级呈正相关（r = 0.776）。反之，CD34 免疫反应性的面积百分比与肿瘤分级也呈正相关（r = 0.690）。Pearson 相关性分析显示 TAMs 与 MVD 之间存在正相关关系（r = 0.680；P <.001）。