年轻和老年口腔鳞状细胞癌患者表现出相似的血管生成特征和 M2 巨噬细胞占优势：比较免疫组织化学研究。
Young and elderly oral squamous cell carcinoma patients present similar angiogenic profile and predominance of M2 macrophages: Comparative immunohistochemical study.
机构信息
Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School (FMRP/USP), University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Hematology Division, Department of Clinical Medicine, Ribeirão Preto Medical School (FMRP/USP), University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
出版信息
Head Neck. 2019 Dec;41(12):4111-4120. doi: 10.1002/hed.25954. Epub 2019 Sep 9.
BACKGROUND
M2 macrophages are often detected in oral squamous cell carcinoma (OSCC), which, influenced by hypoxic conditions, appear to have high angiogenesis-inducing capacity. However, the effects of immunosenescence on tumor-associated macrophages (TAMs) and angiogenesis in OSCC are unknown.
METHODS
Fifty-seven OSCCs were divided into 3 groups (I: <40 years [n = 17]; II: 40-65 years [n = 20]; III: >65 years [n = 20]). Immunohistochemistry for CD68 and CD163 (TAMs), and CD34 and D2-40 for microvessel density (MVD), microvessel area (MVA), and total vascular area (TVA) were performed.
RESULTS
All groups showed similar clinicopathological and immunohistochemical findings. Similar CD68 and CD163 expression, confirmed a M2 phenotype. MVD, MVA, and TVA were similar, however, with significant predominance of blood vessels. No significant correlation between macrophage and angiogenic markers was observed.
CONCLUSIONS
A similar TAM and angiogenesis profile suggests the participation of other mechanisms, instead immunosenescence, in young and elderly OSCC patients.
背景
M2 巨噬细胞常在口腔鳞状细胞癌（OSCC）中被检测到，受缺氧条件影响，M2 巨噬细胞似乎具有较高的血管生成诱导能力。然而，免疫衰老对 OSCC 中肿瘤相关巨噬细胞（TAMs）和血管生成的影响尚不清楚。
方法
将 57 例 OSCC 分为 3 组（I：<40 岁[n=17]；II：40-65 岁[n=20]；III：>65 岁[n=20]）。采用 CD68 和 CD163（TAMs）的免疫组织化学染色，以及 CD34 和 D2-40 检测微血管密度（MVD）、微血管面积（MVA）和总血管面积（TVA）。
结果
所有组均显示出相似的临床病理和免疫组织化学发现。相似的 CD68 和 CD163 表达证实了 M2 表型。MVD、MVA 和 TVA 相似，但血管占明显优势。未观察到巨噬细胞和血管生成标志物之间存在显著相关性。
结论
相似的 TAM 和血管生成特征表明，在年轻和老年 OSCC 患者中，免疫衰老以外的其他机制参与其中。
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