Smith R C, Neff A W
Anat Rec. 1985 Dec;213(4):518-9, 536-7. doi: 10.1002/ar.1092130407.
Addition of 0.5 microgram/ml of the red fluorescent dye propidium iodide to the penultimate wash of Xenopus early embryo serial sections during immunofluorescence studies causes germ plasm to fluoresce brightly. Germ plasm can be accurately and speedily identified in the serial sections. The use of this dye is a marked improvement over previous methods of germ plasm identification in unstained sections. Studies involving screening for germ-plasm-specific antibodies are greatly facilitated by staining germ plasm red with propidium iodide and searching for green colabelling by fluoresceinated antibodies.
在免疫荧光研究中，向非洲爪蟾早期胚胎连续切片的倒数第二次洗涤液中添加0.5微克/毫升的红色荧光染料碘化丙啶，可使生殖质发出明亮荧光。在连续切片中能够准确且快速地识别出生殖质。与之前在未染色切片中识别生殖质的方法相比，这种染料的使用有了显著改进。通过用碘化丙啶将生殖质染成红色，并寻找荧光素化抗体的绿色共标记，极大地促进了涉及筛选生殖质特异性抗体的研究。
