Food Process Engineering Lab, Department of Chemical Engineering, A.C.Tech Campus, Anna University, Chennai 600025, Tamil Nadu, India.
Food Process Engineering Lab, Department of Chemical Engineering, A.C.Tech Campus, Anna University, Chennai 600025, Tamil Nadu, India.
Int J Biol Macromol. 2024 Oct;277(Pt 3):134295. doi: 10.1016/j.ijbiomac.2024.134295. Epub 2024 Aug 6.
Complete valorization of lignocellulosic biomass is crucial for bio-based biorefineries to fulfil the circular bioeconomy concept. However, the existence of lignin carbohydrate complexes (LCC) in biomass hinders the simultaneous fractionation of biomass components, such as lignin, hemicellulose and cellulose, for subsequent biorefining processes. This study explores for the first time a novel approach tailored for the deconstruction of sorghum biomass components through efficient breakdown of LCC. Selective targeting of the major LCC linkages binding xylan and lignin was performed using an ultrasound-assisted deep eutectic solvent under mild treatment conditions. This process yielded a maximum cellulose content of 98.3 %, hemicellulose content of 95.2 %, and lignin content of 94.6 %, with the highest purities of 99.43 %, 96.71 %, and 98.12 %, respectively. FTIR, 2D-HSQC NMR and XRD analyses confirmed that most of the structural properties of lignin, hemicellulose, cellulose are retained. The lignocellulosic components were successfully valorised to cellulose, hemicellulose, and lignin nanoparticles with mean sizes of 64.5 ± 6 nm, 72.8 ± 4 nm and 57.2 ± 8 nm respectively, with good thermal stability. The proposed green process enables the complete utilization of agro-residue feedstock for the preparation of biomass-derived nanoparticles, thereby accelerating the economic and industrial prospects of bio-based biorefineries.
木质纤维素生物质的完全利用对于基于生物的生物精炼厂实现循环生物经济概念至关重要。然而，生物质中木质素碳水化合物复合物（LCC）的存在阻碍了生物质成分（如木质素、半纤维素和纤维素）的同时分级，从而阻碍了后续的生物精炼过程。本研究首次探索了一种通过有效分解 LCC 来解构高粱生物质成分的新方法。在温和的处理条件下，使用超声辅助深共晶溶剂对主要结合木聚糖和木质素的 LCC 键进行选择性靶向断裂。该工艺可获得纤维素含量最高为 98.3%、半纤维素含量最高为 95.2%、木质素含量最高为 94.6%，纯度最高分别为 99.43%、96.71%和 98.12%。FTIR、2D-HSQC NMR 和 XRD 分析证实，木质素、半纤维素、纤维素的大部分结构特性得以保留。木质纤维素成分成功地转化为纤维素、半纤维素和木质素纳米颗粒，平均粒径分别为 64.5±6nm、72.8±4nm 和 57.2±8nm，具有良好的热稳定性。所提出的绿色工艺可实现农业残余原料的完全利用，用于制备生物质衍生的纳米颗粒，从而加速基于生物的生物精炼厂的经济和工业前景。
