One pot green process for facile fractionation of sorghum biomass to lignin, cellulose and hemicellulose nanoparticles using deep eutectic solvent.

Complete valorization of lignocellulosic biomass is crucial for bio-based biorefineries to fulfil the circular bioeconomy concept. However, the existence of lignin carbohydrate complexes (LCC) in biomass hinders the simultaneous fractionation of biomass components, such as lignin, hemicellulose and cellulose, for subsequent biorefining processes. This study explores for the first time a novel approach tailored for the deconstruction of sorghum biomass components through efficient breakdown of LCC. Selective targeting of the major LCC linkages binding xylan and lignin was performed using an ultrasound-assisted deep eutectic solvent under mild treatment conditions. This process yielded a maximum cellulose content of 98.3 %, hemicellulose content of 95.2 %, and lignin content of 94.6 %, with the highest purities of 99.43 %, 96.71 %, and 98.12 %, respectively. FTIR, 2D-HSQC NMR and XRD analyses confirmed that most of the structural properties of lignin, hemicellulose, cellulose are retained. The lignocellulosic components were successfully valorised to cellulose, hemicellulose, and lignin nanoparticles with mean sizes of 64.5 ± 6 nm, 72.8 ± 4 nm and 57.2 ± 8 nm respectively, with good thermal stability. The proposed green process enables the complete utilization of agro-residue feedstock for the preparation of biomass-derived nanoparticles, thereby accelerating the economic and industrial prospects of bio-based biorefineries.