Guo Xuanyue, Yang Zhen, Zhang Jinjin, Hua Juan, Luo Shihong
Engineering Research Center of Protection and Utilization of Plant Resources, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, China.
Front Plant Sci. 2024 Jul 19;15:1369074. doi: 10.3389/fpls.2024.1369074. eCollection 2024.
The adaptation of pathogenic fungi to plant-specialized metabolites is necessary for their survival and reproduction. The biotrophic fungus can cause maize smut and produce tumors in maize (), resulting in reduced maize yield and significant economic losses. Qualitative analysis using UPLC-MS/MS revealed that the infection of maize variety B73 with resulted in increased levels of phytohormones, phenolics, and alkaloids in maize seedling tissues. However, correlation analysis showed that nearly all compounds in the mechanical damage group were significantly negatively correlated with the shoot growth indexes of maize B73. The correlation coefficients of 2-hydroxy-7-methoxy-1,4-benzoxazin-3-one (HMBOA) and maize B73 shoot length and shoot weight were = -0.56 ( < 0.01) and = -0.75 ( < 0.001), respectively. In the inoculation group, these correlations weakened, with the correlation coefficients between HMBOA and maize B73 shoot length and shoot weight being = 0.02 and = -0.1, respectively. The correlation coefficients between 6-methoxy-2-benzoxazolinone (MBOA) and the shoot weight were = -0.73 ( < 0.001) and = -0.15 in the mechanical damage group and inoculation group, respectively. These findings suggest that increased concentrations of these compounds are more positively associated with mechanical damage than with infection. At high concentrations, most of these compounds had an inhibitory effect on . This study investigated the ability of to regulate various compounds, including phytohormones, phenolic acids, and alkaloids in maize B73, providing evidence that has adapted to the specialized metabolites produced by maize B73.
病原真菌对植物特化代谢产物的适应对其生存和繁殖至关重要。活体营养型真菌可引发玉米黑粉病并在玉米中产生肿瘤（），导致玉米产量下降和重大经济损失。使用超高效液相色谱-串联质谱法（UPLC-MS/MS）进行的定性分析表明，用感染玉米品种B73会导致玉米幼苗组织中植物激素、酚类和生物碱水平升高。然而，相关性分析表明，机械损伤组中几乎所有化合物与玉米B73的地上部生长指标均呈显著负相关。2-羟基-7-甲氧基-1,4-苯并恶唑-3-酮（HMBOA）与玉米B73地上部长度和地上部重量的相关系数分别为 = -0.56（<0.01）和 = -0.75（<0.001）。在接种组中，这些相关性减弱，HMBOA与玉米B73地上部长度和地上部重量之间的相关系数分别为 = 0.02和 = -0.1。6-甲氧基-2-苯并恶唑啉酮（MBOA）与地上部重量的相关系数在机械损伤组和接种组中分别为 = -0.73（<0.001）和 = -（此处原文似乎有误，推测可能是 = -0.15）。这些发现表明，这些化合物浓度的增加与机械损伤的正相关性比与感染的正相关性更强。在高浓度下，这些化合物中的大多数对有抑制作用。本研究调查了在玉米B73中调节各种化合物（包括植物激素、酚酸和生物碱）的能力，提供了已适应玉米B73产生的特化代谢产物的证据。
