胰岛素和伴刀豆球蛋白A对大鼠肝癌细胞中特定mRNA积累的影响。

The effects of insulin and concanavalin A on the accumulation of a specific mRNA in rat hepatoma cells.

作者信息

Messina J L, Hamlin J, Azizkahn J, Larner J

出版信息

Biochem Biophys Res Commun. 1985 Dec 31;133(3):1168-74. doi: 10.1016/0006-291x(85)91259-8.

Abstract

One of insulin's effects is to stimulate specific mRNA synthesis. Treatment of H4IIE hepatoma cells with 0.01-1.0 nM insulin results in a maximum 10-15 fold increase in the accumulation of a specific mRNA (p33-mRNA) as measured with a cloned cDNA. Concanavalin A, a lectin known to mimic many of insulin's effects, also stimulates the accumulation of p33-mRNA. The effects of both insulin and Con A were blocked by the addition of two RNA synthesis inhibitors, actinomycin D or 5,6 dichloro-1-beta-D-ribofuranosyl-benzimidazole. We therefore suggest that insulin and concanavalin A act to stimulate p33-mRNA synthesis.

摘要

胰岛素的作用之一是刺激特定的mRNA合成。用0.01 - 1.0 nM胰岛素处理H4IIE肝癌细胞，会使一种特定mRNA（p33 - mRNA）的积累量最多增加10 - 15倍，这是通过克隆cDNA测量得出的。伴刀豆球蛋白A是一种已知能模拟胰岛素许多作用的凝集素，它也能刺激p33 - mRNA的积累。添加两种RNA合成抑制剂放线菌素D或5,6 - 二氯 - 1 - β - D - 呋喃核糖基苯并咪唑可阻断胰岛素和伴刀豆球蛋白A的作用。因此，我们认为胰岛素和伴刀豆球蛋白A的作用是刺激p33 - mRNA的合成。