Microplastics contamination in the most popular brands of Iranian sausages and evaluation of its human exposure.

Microplastics (MPs) pollution represents a nascent environmental contaminant that has recently infiltrated human life and the food chain. The primary objective of this study was to investigate the presence of MPs in different brands of Iranian sausages. Qualitative and quantitative analyses of MPs particles were conducted using stereo- and fluorescent microscopy, FT-IR (Fourier-transform infrared spectroscopy), and SEM-EDS (Scanning electron microscopy-energy dispersive X-ray spectroscopy) techniques. Samples were collected from the most commonly consumed sausage brands in Iranian markets. The findings showed that the various sausage brands contained an average abundance of 25.7 ± 21.68 (range 10-70) and 55.45 ± 45.5 (range 10-175) particles/kg based on optical and fluorescent microscopy analyses, respectively. Predominantly, MPs were identified in fiber form (77-89 %), with a smaller proportion present in fragmented form (11-23 %). Polymer analysis using FT-IR identified polyethylene (PE) and polystyrene (PS) as the primary constituents. Furthermore, the estimated annual intake (EAI) of MPs was calculated at 804 and 3517 particles/kg bw/year for adults and children, respectively, based on optical microscopy observations. In comparison, fluorescent microscopy indicated an intake of 1734 and 7589 particles/kg bw/year for the respective age groups. These results emphasize the potential of MPs contamination to penetrate into different food products including sausages through processing routes, which can threaten human health.