Alshammari Ayed, Subhani Muhammad Irshad, H Wakid Majed, A M Alkhaldi Abdulsalam, Hussain Shujaat, Malik Muhammad Abdullah, Saqib Muhammad, Qamar Warda, Alvi Mughees Aizaz
Department of Biology, College of Science, University of Hafr Al Batin, Hafr Al Batin, Saudi Arabia.
Authors contributed equally to this work.
J Adv Vet Anim Res. 2024 Jun 4;11(2):264-274. doi: 10.5455/javar.2024.k772. eCollection 2024 Jun.
Alveolar echinococcosis is caused by , a parasite of zoonotic significance with a wide range of intermediate and final hosts, and the parasite survives successfully in diversified conditions. Plentiful studies have been done to study the genetic structure of the population of the parasite and the level of intimate kinship using mitochondrial (mt) DNA. The present study was conducted to investigate the population structure, genetic variation, and phylogenetic relationship of various isolates of submitted to GenBank worldwide. Sequences of mt genes (mt-cytochrome c oxidase (1), mt-NADH dehydrogenase 1)) of were analyzed to achieve the set goals.
A total of 275 and 124 gene sequences of mt-1 and mt-1 belonging to . respectively, were retrieved from the National Center for Biotechnology Information GenBank. The retrieved sequences were subjected to alignment with respective reference sequences using MEGA software. The PopArt software was used to establish median-joining networks, while DnaSp was used to calculate neutrality and diversity indices. MrBayes software was used to investigate the phylogenetic association between haplotypes based on Bayesian phylogeny.
Approximately 13 and 20 distinctive haplotypes of 1 and 1 genes, respectively, were observed in the present study. In both of the mt genes, diversity indices indicated low haplotype (mt-1 = 0.140; mt-1 = 0.374) and nucleotide (mt-1 = 0.00111; mt-1 = 0.00287) diversities. The values of Tajima's D and Fu Fs for a population of both of the genes under study were found to be negative.
This study is a maiden attempt to provide insights into the population structure and genetic variation of on a global scale. However, it is suggested that to better understand the population structure and genetic diversity of . more geographical locations and amplifications of full-length gene sequences should be considered, which could be helpful in widening the insights into the genetic diversity of . .
肺泡型棘球蚴病由一种具有人畜共患病意义的寄生虫引起，该寄生虫有广泛的中间宿主和终末宿主，且能在多种条件下成功存活。已经开展了大量研究，利用线粒体（mt）DNA来研究该寄生虫种群的遗传结构和近亲关系水平。本研究旨在调查提交至GenBank的世界各地不同株的种群结构、遗传变异和系统发育关系。分析了该寄生虫的mt基因（mt-细胞色素c氧化酶（1）、mt-NADH脱氢酶1））序列以实现既定目标。
分别从美国国立生物技术信息中心GenBank中检索到总共275条和124条属于该寄生虫的mt-1和mt-1基因序列。使用MEGA软件将检索到的序列与各自的参考序列进行比对。使用PopArt软件构建中介连接网络，而DnaSp用于计算中性和多样性指数。使用MrBayes软件基于贝叶斯系统发育来研究单倍型之间的系统发育关联。
在本研究中分别观察到该寄生虫的mt-1和mt-1基因约13种和20种不同的单倍型。在这两个mt基因中，多样性指数均表明单倍型多样性低（mt-1 = 0.140；mt-1 = 0.374）和核苷酸多样性低（mt-1 = 0.00111；mt-1 = 0.00287）。在所研究的两个基因的种群中，Tajima's D和Fu Fs值均为负数。
本研究首次尝试在全球范围内深入了解该寄生虫的种群结构和遗传变异。然而，建议为了更好地理解该寄生虫的种群结构和遗传多样性，应考虑更多地理位置以及全长基因序列的扩增，这可能有助于拓宽对该寄生虫遗传多样性的认识。
