南非杜泊绵羊第2外显子中基因的遗传变异

Genetic variations of gene in exon 2 of South African Dorper sheep.

作者信息

Selala Lebelo, Chitura Teedzai, Mbazima Vusi, Tyasi Louis

机构信息

Department of Agricultural Economics and Animal Production, University of Limpopo, Polokwane, South Africa.

Department of Animal Science, University of Venda, Thohoyandou, South Africa.

出版信息

J Adv Vet Anim Res. 2024 Jun 8;11(2):302-305. doi: 10.5455/javar.2024.k777. eCollection 2024 Jun.

DOI:10.5455/javar.2024.k777

PMID:39101091

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11296168/

Abstract

OBJECTIVE

The study was conducted to identify the sequence variation of ( in exon 2 of South African Dorper sheep.

MATERIALS AND METHODS

Blood samples were collected from fifty ( 50) South African Dorper sheep aged between 3 and 4 years. The Deoxyribonucleic acid (DNA) was extracted, amplified, and sequenced for the gene. DNA sequencing was used to identify the sequence variations of the gene in South African Dorper sheep.

RESULTS

The results showed that one synonymous single nucleotide polymorphism (SNP) of the gene in exon 2 position T2249C was identified. Two genotypes (TT and TC) were discovered from the identified SNP. The dominant genotype was TT (0.60) over TC (0.40), with the dominant allele T (0.80) over C (0.20). The results also indicated that the used population was in the Hady-Weinberg Equilibrium. Polymorphism genetic analysis findings suggest that the identified sequence variation of TLR4 in exon 2 of South African Dorper sheep was moderate polymorphism.

CONCLUSION

gene at exon 2 of South African Dorper sheep had the SNP (T>C) at position 2249 bp with two genotypes (TT and TC).

摘要

目的

本研究旨在鉴定南非杜泊绵羊（）外显子2中的序列变异。

材料与方法

采集50只年龄在3至4岁之间的南非杜泊绵羊的血样。提取、扩增并对该基因进行测序。采用DNA测序法鉴定南非杜泊绵羊中该基因的序列变异。

结果

结果显示，在该基因外显子2位置T2249C处鉴定出一个同义单核苷酸多态性（SNP）。从鉴定出的SNP中发现了两种基因型（TT和TC）。优势基因型为TT（0.60），高于TC（0.40），优势等位基因为T（0.80），高于C（0.20）。结果还表明，所使用的群体处于哈迪-温伯格平衡状态。多态性遗传分析结果表明，南非杜泊绵羊外显子2中鉴定出的TLR4序列变异为中度多态性。