transmission based on merozoite surface protein 1 () and 2 () gene diversity and antibody responses in Ibadan, Nigeria.

BACKGROUND

Nigeria is a major contributor to the global malaria burden. The genetic diversity of malaria parasite populations as well as antibody responses of individuals in affected areas against antigens of the parasite can reveal the transmission intensity, a key information required to control the disease. This work was carried out to determine the allelic frequency of highly polymorphic genes and antibody responses against schizont crude antigens in an area of Ibadan, Nigeria.

MATERIALS AND METHODS

Blood was collected from 147 individuals with symptoms suspected to be malaria. Malaria infection was determined using a rapid diagnostic test (RDT), and and were genotyped by a nested PCR method. In addition, levels of IgG directed against FCR3S1.2 schizont extract was measured in ELISA.

RESULTS

Approximately 25% (36/147) were positive for a infection in RDT, but only 32 of the positive samples were successfully genotyped. MAD20 was the most prevalent and K1 the least prevalent of the alleles. For , FC27 was more prevalent than 3D7. The mean multiplicities of infection (MOI) were 1.9 and 1.7 for and , respectively. IgG levels correlated positively with age, however there was no difference in median antibody levels between RDT-positive and RDT-negative individuals.

CONCLUSION

Low MOI has before been correlated with low/intermediate transmission intensity, however, in this study, similar levels of -specific antibodies between infected and non-infected individuals point more towards a high level of exposure and a need for further measures to control the spread of malaria in this area.