Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, 11884, Egypt.
Biology Department, College of Sciences, King Khalid University, Abha, Saudi Arabia.
Sci Rep. 2024 Aug 5;14(1):18052. doi: 10.1038/s41598-024-67233-0.
The novelty of this study lies in demonstrating a new approach to control wilt diseases using Jania ethyl acetate extract. In the current investigation, the potential impacts of Jania sp. ethyl acetate extract (JE) on Tomato Fusarium oxysporum wilt (FOW) have been studied. The in vitro antifungal potential of JE against F. oxysporum (FO) was examined. GC-MS investigation of the JE revealed that, the compounds possessing fungicidal action were Phenol,2-methoxy-4-(2-propenyl)-,acetate, Eugenol, Caryophyllene oxide, Isoespintanol, Cadinene, Caryophylla-4(12),8(13)-dien-5à-ol and Copaen. Jania sp. ethyl acetate extract exhibited strong antifungal potential against FO, achieving a 20 mmzone of inhibition. In the experiment, two different methods were applied: soil irrigation (SI) and foliar application (FS) of JE. The results showed that both treatments reduced disease index present DIP by 20.83% and 33.33% respectively. The findings indicated that during FOW, proline, phenolics, and the antioxidant enzymes activity increased, while growth and photosynthetic pigments decreased. The morphological features, photosynthetic pigments, total phenol content, and antioxidant enzyme activity of infected plants improved when JE was applied through soil or foliar methods. It is interesting to note that the application of JE had a substantially less negative effect on the isozymes peroxidase and polyphenol oxidase in tomato plants, compared to FOW. These reactions differed depending on whether JE was applied foliarly or via the soil. Finally, the use of Jania sp. could be utilized commercially as an ecologically acceptable method to protect tomato plants against FOW.
本研究的新颖之处在于展示了一种使用乙酸乙酯提取物控制枯萎病的新方法。在目前的研究中，研究了 Jania sp. 乙酸乙酯提取物（JE）对番茄尖孢镰刀菌枯萎病（FOW）的潜在影响。研究了 JE 对 F. oxysporum（FO）的体外抑菌作用。JE 的 GC-MS 研究表明，具有杀菌作用的化合物是酚，2-甲氧基-4-（2-丙烯基）-，乙酸酯，丁香酚，石竹烯氧化物，异桉醇，卡丹烯，石竹-4（12），8（13）-二烯-5α-醇和古巴烯。Jania sp. 乙酸乙酯提取物对 FO 表现出很强的抑菌潜力，抑制圈达到 20mm。在实验中，应用了两种不同的方法：JE 的土壤灌溉（SI）和叶面喷施（FS）。结果表明，两种处理均使病情指数分别降低了 20.83%和 33.33%。结果表明，在 FOW 期间，脯氨酸、酚类和抗氧化酶活性增加，而生长和光合色素减少。通过土壤或叶面法施用 JE 可改善感病植物的形态特征、光合色素、总酚含量和抗氧化酶活性。有趣的是，与 FOW 相比，JE 对番茄植株同工酶过氧化物酶和多酚氧化酶的负面影响要小得多。这些反应因 JE 是叶面喷施还是土壤施用而有所不同。最后，Jania sp. 的使用可以作为一种生态上可接受的方法，用于商业上保护番茄植物免受 FOW 的侵害。
