Idowu Modoluwamu, Taiwo Godstime, Sidney Taylor, Adewoye Anjola, Ogunade Ibukun M
Division of Animal Science, West Virginia University, Morgantown, WV, United States.
Department of Chemistry, West Virginia University, Morgantown, WV, United States.
Front Vet Sci. 2024 Jul 22;11:1415594. doi: 10.3389/fvets.2024.1415594. eCollection 2024.
We utilized plasma proteomics profiling to explore metabolic pathways and key proteins associated with divergent residual body weight gain (RADG) phenotype in crossbred (Angus × Hereford) beef steers. A group of 108 crossbred growing beef steers (average BW = 282.87 ± 30 kg; age = 253 ± 28 days) were fed a high-forage total mixed ration for 49 days in five dry lot pens (20-22 beef steers per pen), each equipped with two GrowSafe8000 intake nodes to determine their RADG phenotype. After RADG identification, blood samples were collected from the beef steers with the highest RADG (most efficient; = 15; 0.76 kg/d) and lowest RADG (least efficient; = 15; -0.65 kg/d). Plasma proteomics analysis was conducted on all plasma samples using a nano LC-MS/MS platform. Proteins with FC ≥ 1.2 and false-discovery rate-adjusted values (FDR) ≤ 0.05 were considered significantly differentially abundant. The analysis identified 435 proteins, with 59 differentially abundant proteins (DAPs) between positive and negative-RADG beef steers. Plasma abundance of 38 proteins, such as macrophage stimulating 1 and peptidase D was upregulated (FC ≥ 1.2, FDR ≤ 0.05) in positive-RADG beef steers, while 21 proteins, including fibronectin and ALB protein were greater (FC < 1.2, FDR ≤ 0.05) in negative-RADG beef steers. The results of the Gene Ontology (GO) analysis of all the DAPs showed enrichment of pathways such as metabolic processes, biological regulation, and catalytic activity in positive-RADG beef steers. Results of the EuKaryotic Orthologous Groups (KOG) analysis revealed increased abundance of DAPs involved in energy production and conversion, amino acid transport and metabolism, and lipid transport and metabolism in positive-RADG beef steers. The results of this study revealed key metabolic pathways and proteins associated with divergent RADG phenotype in beef cattle which give more insight into the biological basis of feed efficiency in crossbred beef cattle.
我们利用血浆蛋白质组学分析来探索杂交（安格斯×赫里福德）肉牛中与不同剩余体重增加（RADG）表型相关的代谢途径和关键蛋白质。一组108头杂交生长肉牛（平均体重=282.87±30千克；年龄=253±28天）在五个干栏舍（每个栏舍20 - 22头肉牛）中饲喂高粗饲料全混合日粮49天，每个栏舍配备两个GrowSafe8000采食节点以确定它们的RADG表型。在确定RADG后，从RADG最高（最有效率；n = 15；0.76千克/天）和最低（最无效率；n = 15；-0.65千克/天）的肉牛中采集血样。使用纳升液相色谱 - 串联质谱平台对所有血浆样本进行血浆蛋白质组学分析。倍数变化（FC）≥1.2且错误发现率调整值（FDR）≤0.05的蛋白质被认为是显著差异丰富的。分析鉴定出435种蛋白质，其中在正、负RADG肉牛之间有59种差异丰富蛋白质（DAPs）。在正RADG肉牛中，38种蛋白质（如巨噬细胞刺激蛋白1和肽酶D）的血浆丰度上调（FC≥1.2，FDR≤0.05），而在负RADG肉牛中，包括纤连蛋白和ALB蛋白在内的21种蛋白质丰度更高（FC < 1.2，FDR≤0.05）。所有DAPs的基因本体（GO）分析结果表明，正RADG肉牛中代谢过程、生物调节和催化活性等途径显著富集。真核直系同源组（KOG）分析结果显示，正RADG肉牛中参与能量产生和转化、氨基酸转运和代谢以及脂质转运和代谢的DAPs丰度增加。本研究结果揭示了与肉牛不同RADG表型相关的关键代谢途径和蛋白质，这为深入了解杂交肉牛饲料效率的生物学基础提供了更多信息。
