Hou Aiqin, Fu Huayi, Liu Leilei, Su Xiaomei, Zhang Shusheng, Lai Jiahou, Sun Faqian
College of Geography and Environmental Science, Zhejiang Normal University, Jinhua, China.
The Management Center of Wuyanling National Natural Reserve in Zhejiang, Wenzhou, China.
Front Microbiol. 2024 Jul 22;15:1433046. doi: 10.3389/fmicb.2024.1433046. eCollection 2024.
Water reservoir sediments represent a distinct habitat that harbors diverse microbial resources crucial for nitrogen cycling processes. The discovery of resuscitation promoting factor (Rpf) has been recognized as a crucial development in understanding the potential of microbial populations. However, our understanding of the relationship between microorganisms containing -like genes and nitrogen-cycling functional populations remains limited. The present study explored the distribution patterns of -like genes and nitrogen-cycling genes in various water reservoir sediments, along with their correlation with environmental factors. Additionally, the co-occurrence of -like genes with genes associated with the nitrogen cycle and viable but non-culturable (VBNC) formation was investigated. The findings indicated the ubiquitous occurrence of Rpf-like domains and their related genes in the examined reservoir sediments. Notably, -like genes were predominantly associated with , , and , with pH emerging as the primary influencing factor for their distribution. Genera such as , , , and harbor the majority of nitrogen-cycling functional genes, particularly denitrification genes. The distribution of nitrogen-cycling microbial communities in the reservoir sediments was mainly influenced by pH and NH . Notably, correlation network analysis revealed close connections between microorganisms containing -like genes and nitrogen-cycling functional populations, as well as VBNC bacteria. These findings offer new insights into the prevalence of -like genes in the water reservoir sediments and their correlation with nitrogen-cycling microbial communities, enhancing our understanding of the significant potential of microbial nitrogen cycling.
水库沉积物代表了一个独特的栖息地，其中蕴藏着对氮循环过程至关重要的多样微生物资源。复苏促进因子（Rpf）的发现被认为是理解微生物种群潜力方面的一项关键进展。然而，我们对含有类似基因的微生物与氮循环功能种群之间关系的理解仍然有限。本研究探索了各种水库沉积物中类似基因和氮循环基因的分布模式，以及它们与环境因素的相关性。此外，还研究了类似基因与氮循环相关基因以及活的但不可培养（VBNC）形成的共现情况。研究结果表明，在所检测的水库沉积物中普遍存在Rpf样结构域及其相关基因。值得注意的是，类似基因主要与、和相关，pH值成为其分布的主要影响因素。诸如、、和等属拥有大多数氮循环功能基因，特别是反硝化基因。水库沉积物中氮循环微生物群落的分布主要受pH值和NH的影响。值得注意的是，相关网络分析揭示了含有类似基因的微生物与氮循环功能种群以及VBNC细菌之间的紧密联系。这些发现为水库沉积物中类似基因的普遍性及其与氮循环微生物群落的相关性提供了新的见解，增强了我们对微生物氮循环巨大潜力的理解。
