Key Laboratory of Soybean Biology in Chinese Ministry of Education (Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry), Northeast Agricultural University, Harbin 150030, China.
Daqing Branch, Heilongjiang Academy of Agricultural Science, Daqing 163711, China.
J Agric Food Chem. 2024 Aug 21;72(33):18573-18584. doi: 10.1021/acs.jafc.4c03012. Epub 2024 Aug 6.
Isoflavone is a secondary metabolite of the soybean phenylpropyl biosynthesis pathway with physiological activity and is beneficial to human health. In this study, the isoflavone content of 205 soybean germplasm resources from 3 locations in 2020 showed wide phenotypic variation. A joint genome-wide association study (GWAS) and weighted gene coexpression network analysis (WGCNA) identified 33 single-nucleotide polymorphisms and 11 key genes associated with soybean isoflavone content. Gene ontology enrichment analysis, gene coexpression, and haplotype analysis revealed natural variations in the 12109800 (7) gene and promoter region, with Hap1 being the elite haplotype. Transient overexpression and knockout of 7 increased and decreased the isoflavone content, respectively, in hairy roots. The combination of GWAS and WGCNA effectively revealed the genetic basis of soybean isoflavone and identified potential genes affecting isoflavone synthesis and accumulation in soybean, providing a valuable basis for the functional study of soybean isoflavone.
异黄酮是大豆苯丙氨酸生物合成途径的次生代谢物，具有生理活性，有益于人类健康。本研究对 2020 年来自 3 个地点的 205 份大豆种质资源的异黄酮含量进行了研究，结果表明其表型变异广泛。联合全基因组关联研究（GWAS）和加权基因共表达网络分析（WGCNA）鉴定出与大豆异黄酮含量相关的 33 个单核苷酸多态性和 11 个关键基因。基因本体富集分析、基因共表达和单倍型分析揭示了 12109800（7）基因及其启动子区域的自然变异，其中 Hap1 是优势单倍型。7 的瞬时过表达和敲除分别增加和减少了毛状根中的异黄酮含量。GWAS 和 WGCNA 的联合应用有效地揭示了大豆异黄酮的遗传基础，并鉴定出了可能影响大豆异黄酮合成和积累的关键基因，为大豆异黄酮的功能研究提供了有价值的依据。
