Department of Animal Biotechnology and Genetics, Faculty of Animal Breeding and Biology, Bydgoszcz University of Science and Technology, Bydgoszcz 85-084, Poland.
Department of Animal Breeding and Nutrition, Faculty of Animal Breeding and Biology, Bydgoszcz University of Science and Technology, Bydgoszcz 85-084 Poland.
Poult Sci. 2024 Oct;103(10):104108. doi: 10.1016/j.psj.2024.104108. Epub 2024 Jul 17.
A healthy gut is one of the main factors influencing bird response. Over the years, efforts have been made to improve intestinal health. One of the supporting methods may be enriching the diet with bioactive ingredients, including sodium butyrate (SB). One of the possible ways of administering such supplementation is in ovo technology. Over the years, research has shown that administering bioactive substances this way has a positive effect on the health status of chickens. The current study aimed to modify the gut microbiota of broiler chickens by in ovo stimulation on d 12 of egg incubation with SB and to determine the changes occurring in intestines. One thousand eggs were incubated and injected with 0.1, 0.3, or 0.5% SB on d 12 of incubation. The control group was injected with physiological saline. Samples collected for analysis were obtained postmortem from 42-day-old ROSS 308 broiler chickens. Growth performance parameters were also monitored during broiler rearing. Gene expression analysis showed significant changes in the levels of IL4, IFNγ, AvBD1, TJAP and MUC6 genes in the ileum. However, the IL8, MUC2 and MUC6 genes were significantly expressed in the cecal mucosa. These changes depended on the administered dose of butyrate. There was no effect of in ovo administration of various doses of SB on digesta pH, SCFA level and histological parameters. However, a significant increase in Bifidobacterium bacteria was detected in the ileum after administration of a dose of 0.5% SB and in the cecum after administration of a dose of 0.3%. Administration of SB in ovo has the potential to support intestinal health in poultry. The effects depend on the administered dose, while the results indicate a dose of 0.3% as the most optimal.
健康的肠道是影响鸟类反应的主要因素之一。多年来，人们一直致力于改善肠道健康。其中一种支持方法可能是在饮食中添加生物活性成分，包括丁酸钠 (SB)。这种补充的一种可能的给药途径是胚内技术。多年来的研究表明，通过这种方式给予生物活性物质对鸡的健康状况有积极影响。本研究旨在通过在孵化第 12 天用 SB 对肉鸡胚内进行刺激来改变肉鸡的肠道微生物群，并确定肠道中发生的变化。将 1000 个鸡蛋孵化，并在孵化第 12 天注射 0.1%、0.3%或 0.5%的 SB。对照组注射生理盐水。收集用于分析的样本是从 42 日龄 ROSS 308 肉鸡死后获得的。在肉鸡饲养过程中还监测了生长性能参数。基因表达分析显示，在回肠中，IL4、IFNγ、AvBD1、TJAP 和 MUC6 基因的水平发生了显著变化。然而，IL8、MUC2 和 MUC6 基因在盲肠黏膜中表达显著。这些变化取决于丁酸的给药剂量。在胚内给予不同剂量的 SB 对食糜 pH、SCFA 水平和组织学参数没有影响。然而，在回肠中检测到在给予 0.5%SB 剂量后双歧杆菌数量显著增加，在给予 0.3%SB 剂量后盲肠中双歧杆菌数量显著增加。在胚内给予 SB 有潜力支持家禽的肠道健康。效果取决于给药剂量，结果表明 0.3%的剂量最有效。
