Wang Chi, Wang Xing, Yang Zhenbiao, Gao Xiaowei
FAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
iScience. 2024 Jun 28;27(8):110414. doi: 10.1016/j.isci.2024.110414. eCollection 2024 Aug 16.
Cell functions are based on the integrity of actin filaments. The actin cytoskeleton is typically the target but also the source of signals. Arabidopsis PRL1 (Pleiotropic Regulatory Locus 1), regulates multiple cellular processes and physiological responses. However, the precise mechanisms underlying PRL1s multiple functions are unclear. Here, we show that PRL1 maintains actin integrity and concomitant cellular homeostasis. The cortical actin cytoskeleton was de-polymerized in the mutant, causing the developmental root defect. Actin depolymerization, rather than reactive oxygen species (ROS) imbalance, constituted the fundamental cause of retarded root growth in . upregulation by, and cooperation with, actin depolymerization triggered stele cell death in roots. Differential gene expression and alternative splicing defects resulting from actin depolymerization occurred independently in . Our work establishes the cause-effect relationships between actin depolymerization and downstream stress-related signals, revealing a novel function of PRL1 and enhancing the understanding of PRLs functional mechanisms.
细胞功能基于肌动蛋白丝的完整性。肌动蛋白细胞骨架通常是信号的靶点，但也是信号的来源。拟南芥PRL1（多效调控位点1）调节多种细胞过程和生理反应。然而，PRL1多种功能背后的确切机制尚不清楚。在这里，我们表明PRL1维持肌动蛋白的完整性以及随之而来的细胞内稳态。在突变体中，皮层肌动蛋白细胞骨架发生解聚，导致根发育缺陷。肌动蛋白解聚而非活性氧（ROS）失衡是根生长受阻的根本原因。肌动蛋白解聚引起的上调以及与之协同作用触发了PRL1根中中柱细胞死亡。肌动蛋白解聚导致的差异基因表达和可变剪接缺陷在PRL1中独立发生。我们的工作建立了肌动蛋白解聚与下游应激相关信号之间的因果关系，揭示了PRL1的新功能，并加深了对PRL功能机制的理解。
