Sakata H, Yoshida H
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1979 Sep;211(3):221-7. doi: 10.1007/BF02387427.
To demonstrate the cellular localization of prostaglandin E (PGE) in the normal and irritated rabbit iris, the indirect immunofluorescence method was employed. The amounts of PGE1 and PGF2 alpha in the aqueous humor were determined with radioimmunoassay. Immunofluorescence of PGE was observed in the cytoplasm of mesenchymal cells of the iris. PGE-positive cells in the normal iris were scattered in the fibrous stroma, and the shape of these cells was oval to spindle-shaped. The numbers of these cells were remarkably increased in the iridectomized and inflamed iris. The amounts of PGE1 in the aqueous humor was 2.0 +/- 0.53 ng/ml in the normal eyes and 7.92 +/- 2.93 ng/ml in the irritated eyes. The mean level of PGF2 alpha was 0.4 +/- 0.27 ng/ml in the normal eyes and 0.8 +/- 0.58 ng/ml in the irritated eyes.
为了证明前列腺素E（PGE）在正常和受刺激的兔虹膜中的细胞定位，采用了间接免疫荧光法。用放射免疫分析法测定房水中PGE1和PGF2α的含量。在虹膜间充质细胞的细胞质中观察到PGE的免疫荧光。正常虹膜中的PGE阳性细胞散在于纤维基质中，这些细胞的形状为椭圆形至纺锤形。在虹膜切除和发炎的虹膜中，这些细胞的数量显著增加。正常眼房水中PGE1的含量为2.0±0.53 ng/ml，受刺激眼为7.92±2.93 ng/ml。正常眼PGF2α的平均水平为0.4±0.27 ng/ml，受刺激眼为0.8±0.58 ng/ml。
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