State Key Laboratory for Diagnosis & Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis & Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Department of Laboratory Medicine, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Future Microbiol. 2024 Sep;19(13):1111-1117. doi: 10.1080/17460913.2024.2363632. Epub 2024 Aug 7.
Metagenomic next-generation sequencing (mNGS) in diagnosis of human brucellosis is comparatively unexplored. This report details five human brucellosis cases diagnosed using mNGS based on Illumina sequencing platform, comprising three females and two males, four with epidemiological exposure. In cases 1 and 2, plasma mNGS results showed one positive and one negative for , and subsequent blood cultures were both positive. Cases 3, 4 and 5 involved spinal brucellosis, some with paravertebral abscesses. mNGS from infectious tissue samples successfully detected , with read counts ranging between 30 and 1314, yet cultures were negative in cases 4 and 5. Following antibiotic and surgical treatments, all patients showed clinical improvement. This report shows mNGS testing enhances the detection sensitivity of brucellosis diagnosis.
宏基因组下一代测序（mNGS）在人类布鲁氏菌病诊断中的应用尚待探索。本报告详细介绍了基于 Illumina 测序平台，通过 mNGS 诊断的 5 例人类布鲁氏菌病病例，包括 3 名女性和 2 名男性，4 例有流行病学接触史。在病例 1 和 2 中，血浆 mNGS 结果显示 1 例阳性和 1 例阴性，随后的血液培养均为阳性。病例 3、4 和 5 涉及脊椎布鲁氏菌病，部分伴有椎旁脓肿。从感染组织样本中进行 mNGS 成功检测到 ，读长数介于 30 到 1314 之间，而病例 4 和 5 的培养结果为阴性。经过抗生素和手术治疗，所有患者的临床症状均有改善。本报告表明，mNGS 检测可提高布鲁氏菌病诊断的检测灵敏度。
