Jehl F, Monteil H, Gallion C, Thierry R C
Pathol Biol (Paris). 1985 Jun;33(5 Pt 2):511-6.
We describe a rapid and accurate high performance liquid chromatographic (HPLC) method for vancomycin quantitation. This method is then compared with two immunoassays, RIA and FPIA. The chemical extraction step needed for HPLC is simple and rapid. Both conventional reversed phase HPLC and high speed reversed phase HPLC were tested. The mobile phase was a mixture of aqueous ammonium acetate and acetonitrile. Specificity of the HPLC assay was good. Serum levels in 112 clinical specimens assayed by HPLC were regressed against the levels obtained for the same samples by both RIA and FPIA. The correlation was good (RIA : r = 0.945; FPIA : r = 0.967). Considering the disadvantages of RIA, HPLC and FPIA emerge as the methods of choice.
我们描述了一种用于万古霉素定量的快速、准确的高效液相色谱（HPLC）方法。然后将该方法与两种免疫测定法，即放射免疫测定法（RIA）和荧光偏振免疫测定法（FPIA）进行比较。HPLC所需的化学提取步骤简单且快速。对传统反相HPLC和高速反相HPLC都进行了测试。流动相是醋酸铵水溶液和乙腈的混合物。HPLC测定法的特异性良好。通过HPLC测定的112份临床标本中的血清水平与通过RIA和FPIA对相同样本测得的水平进行回归分析。相关性良好（RIA：r = 0.945；FPIA：r = 0.967）。考虑到RIA的缺点，HPLC和FPIA成为首选方法。
