聚乙醇/明胶复合膜的制备、表征、抗菌评价及对葡萄的保鲜应用，其中含有氧化锌@季铵化壳聚糖纳米粒子。

Preparation, characterization, antimicrobial evaluation, and grape preservation applications of polyvinyl alcohol/gelatin composite films containing zinc oxide@quaternized chitosan nanoparticles.

机构信息

Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou 225009, China; Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou University, Yangzhou 225009, China.

出版信息

Int J Biol Macromol. 2024 Oct;277(Pt 4):134527. doi: 10.1016/j.ijbiomac.2024.134527. Epub 2024 Aug 5.

DOI:10.1016/j.ijbiomac.2024.134527

PMID:39111507

Abstract

This study employed a precipitation method to synthesize zinc oxide@quaternised chitosan nanoparticles (ZnO@QAC NPs) containing different concentrations of zinc oxide, namely ZnO@QAC-2, ZnO@QAC-4, and ZnO@QAC-6. Subsequently, these nanoparticles were incorporated into matrices consisting of gelatine (Gn) and polyvinyl alcohol (PVA) separately, which were prepared by casting to form a biodegradable film. We assessed the physicochemical properties of ZnO@QAC NPs and physicochemical characteristics, antioxidant properties, antimicrobial activity and grape preservation efficacy of the film. Compared to the control group, the films showed a reduction in water vapor permeability by >9.38 %, an increase in tensile strength by over 51.95 %, over 70 % scavenging of ABTS free radicals, and good biocompatibility. Additionally, the antimicrobial activity of the films containing ZnO@QAC-6 increased by 37.6 %. In the grape preservation experiment, the weight loss of grapes wrapped in ZnO@QAC-2 film was reduced by 40.13 % on day 15 compared to unwrapped grapes. These results demonstrate that ZnO@QAC/PVA/Gn films have considerable potential for food packaging applications.

摘要

本研究采用沉淀法合成了不同氧化锌浓度的氧化锌@季铵化壳聚糖纳米粒子（ZnO@QAC NPs），分别为 ZnO@QAC-2、ZnO@QAC-4 和 ZnO@QAC-6。随后，将这些纳米粒子分别掺入由明胶（Gn）和聚乙烯醇（PVA）组成的基质中，通过浇铸形成可生物降解的薄膜。我们评估了 ZnO@QAC NPs 的物理化学性质以及薄膜的物理化学特性、抗氧化性能、抗菌活性和葡萄保鲜效果。与对照组相比，薄膜的水蒸气透过率降低了>9.38%，拉伸强度提高了超过 51.95%，ABTS 自由基清除率超过 70%，具有良好的生物相容性。此外，含 ZnO@QAC-6 的薄膜的抗菌活性增加了 37.6%。在葡萄保鲜实验中，与未包装的葡萄相比，用 ZnO@QAC-2 薄膜包裹的葡萄在第 15 天的失重减少了 40.13%。这些结果表明，ZnO@QAC/PVA/Gn 薄膜在食品包装应用方面具有相当大的潜力。