Negishi K, Takahashi M, Nishizawa M, Nomura A, Hayatsu H
Nucleic Acids Symp Ser. 1985(16):237-9.
Molecular mechanism of the mutation induced by N4-aminocytidine was studied. The specificity of in vitro incorporation of N4-aminodeoxycytidine 5'-triphophate catalyzed by E. coli DNA polymerase large fragment was analyzed. The results have shown that this cytosine analog can be efficiently incorporated as a substitute of cytosine, and that it can also be incorporated with a low efficiency as a substitute of thymine. We have also shown that the N4-aminocytosine incorporated opposite adenine can be excised as its monophosphate at a high frequency. The N4-aminocytosine residues in the polynucleotide templates can be read by the enzyme as efficiently as cytosines, and guanines were incorporated opposite them.
研究了N4-氨基胞苷诱导突变的分子机制。分析了大肠杆菌DNA聚合酶大片段催化的N4-氨基脱氧胞苷5'-三磷酸体外掺入的特异性。结果表明，这种胞嘧啶类似物可以作为胞嘧啶的替代物被高效掺入，也可以作为胸腺嘧啶的替代物被低效掺入。我们还表明，与腺嘌呤相对掺入的N4-氨基胞嘧啶可以作为其单磷酸被高频切除。多核苷酸模板中的N4-氨基胞嘧啶残基可以像胞嘧啶一样被酶高效读取，并且鸟嘌呤会与它们相对掺入。
