Zhao Xiaolong, Shang Longqi, Shen Chunjian
Dalian Medical University, No. 9 West Section Lvshun South Road, Dalian 116044, P.R. China.
Department of Nursing, The Second Affiliated Hospital of Shenyang Medical College, No. 20 Beijiu Road, Shenyang, Liaoning Province 110000, P.R. China.
Exp Anim. 2025 Jan 10;74(1):49-57. doi: 10.1538/expanim.24-0027. Epub 2024 Aug 8.
Daphnetin has been demonstrated to exert beneficial effects on diabetes mellitus and renal complications. However, the role and molecular mechanism of daphnetin in diabetic cardiomyopathy (DCM) remain unclear. In this study, rats were injected with streptozotocin (STZ) to induce diabetes. The diabetic rats were then administered daphnetin (1 and 4 mg/kg) or dimethyl sulfoxide (DMSO) daily for 12 weeks. The results demonstrated that the diabetic rats exhibited elevated blood glucose levels, which were dose-dependently ameliorated by daphnetin. At 13 weeks following STZ injection, the rats exhibited typical diabetic signs, cardiac dysfunction, and evident pathological alterations in myocardial tissues. The administration of daphnetin to diabetic rats resulted in improvement in cardiac function, reductions in myocardial injury biomarkers, and the inhibition of myocardial fibrosis. Furthermore, daphnetin treatment suppressed inflammation and endoplasmic reticulum stress-induced apoptosis in a dose-dependent manner. Additionally, daphnetin exhibited partial blockade of the activation of mitogen-activated protein kinase pathways induced by diabetes. These findings indicate that daphnetin may be a promising therapeutic agent for the treatment of DCM.
瑞香素已被证明对糖尿病及其肾脏并发症具有有益作用。然而，瑞香素在糖尿病性心肌病（DCM）中的作用和分子机制仍不清楚。在本研究中，给大鼠注射链脲佐菌素（STZ）以诱导糖尿病。然后，给糖尿病大鼠每天灌胃瑞香素（1和4 mg/kg）或二甲基亚砜（DMSO），持续12周。结果表明，糖尿病大鼠血糖水平升高，而瑞香素能剂量依赖性地改善血糖水平。在注射STZ后13周，大鼠出现典型的糖尿病症状、心脏功能障碍以及心肌组织明显的病理改变。给糖尿病大鼠施用瑞香素可改善心脏功能、降低心肌损伤生物标志物水平并抑制心肌纤维化。此外，瑞香素治疗以剂量依赖性方式抑制炎症和内质网应激诱导的细胞凋亡。此外，瑞香素对糖尿病诱导的丝裂原活化蛋白激酶途径的激活具有部分阻断作用。这些发现表明，瑞香素可能是治疗DCM的一种有前景的治疗药物。
