Department of Medicine, Division of Clinical Immunology, Faculty of Health Sciences HSC 3H2, McMaster University, Hamilton, Ontario, Canada.
Department of Pathology and Molecular Medicine, Faculty of Health Sciences, McMaster University, Hamilton, Ontario, Canada.
Am J Reprod Immunol. 2024 Aug;92(2):e13912. doi: 10.1111/aji.13912.
There is a higher incidence of irritable bowel syndrome with miscarriages, and recurrent miscarriages of otherwise normal embryos have been linked to subnormal expression of the immune checkpoint inhibitor CD200L. We sought to determine if alterations in the expression of the CD200 immune checkpoint inhibitor occur in colonic tissue in IBS-D patients.
Quantitative immunohistochemical staining of biopsies from proximal and distal colon or rectum for the inhibitory CD200L and CD200S molecules was done. CD56 cells were also enumerated as they play a role in recurrent miscarriages and may express CD200S.
CD200L was decreased and CD200S was unchanged in epithelium but not stroma of 3 IBS-D cases. One case had an increase in both CD200L and CD200S. CD56 cells were also stained for CD200S. Degranulation was assessed by the percentage of extracellular CD200S that was increased as epithelial CD200L decreased.
This pilot study was promising and warrants a larger sample to determine if a correlation between uterine implantation site CD200L and CD200S expression in normal and failing implantation sites is needed. Colonic epithelial CD200L may then provide useful information about the pathogenesis of the spontaneous miscarriage in individual cases.
流产患者中肠易激综合征发病率较高，而复发性流产的正常胚胎与免疫检查点抑制剂 CD200L 的表达异常有关。我们试图确定在 IBS-D 患者的结肠组织中是否存在 CD200 免疫检查点抑制剂表达的改变。
对近端和远端结肠或直肠的活检组织进行抑制性 CD200L 和 CD200S 分子的定量免疫组织化学染色。还对 CD56 细胞进行了计数，因为它们在复发性流产中起作用，并且可能表达 CD200S。
3 例 IBS-D 患者的上皮细胞中 CD200L 减少，而 CD200S 不变，但基质中不变。一例患者 CD200L 和 CD200S 均增加。CD56 细胞也被染色用于 CD200S。通过上皮细胞 CD200L 减少时细胞外 CD200S 的增加百分比来评估脱颗粒。
这项初步研究很有前景，需要更大的样本量来确定在正常和失败的着床部位中子宫着床部位 CD200L 和 CD200S 表达是否存在相关性。然后，结肠上皮细胞 CD200L 可能为个别病例中自发性流产的发病机制提供有用信息。
