免疫荧光末端脱氧核苷酸转移酶试剂盒作用的评估

Assessment of the role of an immunofluorescent terminal deoxynucleotidyl transferase kit.

作者信息

Dunbar D R, Smith C, Thornton J A, Waters H M, Hyde K, Delamore I W

出版信息

Scand J Haematol. 1985 Nov;35(5):474-80. doi: 10.1111/j.1600-0609.1985.tb02815.x.

DOI:10.1111/j.1600-0609.1985.tb02815.x

Abstract

The laboratory use of a commercial kit for the immunofluorescent measurement of terminal deoxynucleotidyl transferase (BRL Terminal Deoxynucleotidyl Transferase Immunofluorescent Assay System) has been investigated. The precision of the assay system with stored patient material and its performance with regard to ease of microscopic examination were assessed. The intensity of the fluorescence obtained was found to vary. However, the precision of the assay system on fixed slides stored at -30 degrees C was satisfactory; the method simple, and results obtainable in a relatively short space of time.

摘要

对一种用于末端脱氧核苷酸转移酶免疫荧光测定的商业试剂盒（BRL末端脱氧核苷酸转移酶免疫荧光测定系统）的实验室应用进行了研究。评估了该测定系统对储存的患者样本的精密度及其在显微镜检查便利性方面的性能。发现所获得的荧光强度有所变化。然而，该测定系统在-30℃储存的固定载玻片上的精密度令人满意；方法简单，且能在相对较短的时间内获得结果。